Source:http://linkedlifedata.com/resource/pubmed/id/11152647
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2001-1-26
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| pubmed:abstractText |
Surfactant protein (SP)-B expression is restricted to type II pneumocytes and Clara cells in the lung. Previously, a promoter region of human SP-B gene from -64 to -118 has been identified as critical for the tissue-specific expression of this gene. Two cis-elements for thyroid transcription factor (TTF)-1 and hepatocyte nuclear factor (HNF)-3alpha binding were found within this area. Using an oligonucleotide fragment, we incorporated this region sequence into the promoter of a HIS3 reporter gene in yeast. With this modified yeast a human lung complementary DNA (cDNA) library was screened for DNA-binding proteins, other than TTF-1 and HNF-3alpha, that interacted with this promoter segment. A cDNA clone encoding a novel polypeptide, BR22, was identified that activated the reporter gene expression in yeast. This gene is expressed in many tissues and encodes a protein with bipartite nuclear localization signals. Studies using in vivo yeast two-hybrid analysis, in vitro protein-protein interactions, and coimmunoprecipitation analyses demonstrated that BR22 formed a protein complex with TTF-1. In vivo cotransfection studies further indicated that BR22 could act with TTF-1 to synergistically activate the SP-B promoter in mammalian cells. Our data suggest that BR22 is a TTF-1-associated protein. Through a protein-protein interaction with TTF-1, BR22 can form a complex and activate the human SP-B promoter in vivo.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteolipids,
http://linkedlifedata.com/resource/pubmed/chemical/Pulmonary Surfactants,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/thyroid nuclear factor 1
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
1044-1549
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
24
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
30-37
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:11152647-Amino Acid Sequence,
pubmed-meshheading:11152647-Base Sequence,
pubmed-meshheading:11152647-Binding Sites,
pubmed-meshheading:11152647-Gene Library,
pubmed-meshheading:11152647-Genes, Reporter,
pubmed-meshheading:11152647-Humans,
pubmed-meshheading:11152647-Lung,
pubmed-meshheading:11152647-Nuclear Proteins,
pubmed-meshheading:11152647-Organ Specificity,
pubmed-meshheading:11152647-Precipitin Tests,
pubmed-meshheading:11152647-Promoter Regions, Genetic,
pubmed-meshheading:11152647-Protein Binding,
pubmed-meshheading:11152647-Proteolipids,
pubmed-meshheading:11152647-Pulmonary Surfactants,
pubmed-meshheading:11152647-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:11152647-Sequence Homology, Amino Acid,
pubmed-meshheading:11152647-Transcription Factors,
pubmed-meshheading:11152647-Transcriptional Activation,
pubmed-meshheading:11152647-Transfection,
pubmed-meshheading:11152647-Two-Hybrid System Techniques
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| pubmed:year |
2001
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| pubmed:articleTitle |
BR22, a novel protein, interacts with thyroid transcription factor-1 and activates the human surfactant protein B promoter.
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| pubmed:affiliation |
Department of Internal Medicine, Pulmonary and Critical Care Medicine, UT Southwestern Medical Center, Dallas, Texas 75390-9034, USA. Yih-Shing.Yang@UTSouthwestern.edu.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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