Linked Life Data

11145999

Source:http://linkedlifedata.com/resource/pubmed/id/11145999

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2001-1-23
pubmed:abstractText
Acetylcholine release stimulated by nicotinic agonists was measured as radioactivity released from perfused synaptosomes prepared from mouse interpeduncular nucleus (IPN) that had been loaded with [(3)H]choline. Agonist-stimulated release was dependent upon external calcium and over 90% of released radioactivity was acetylcholine. The release process was characterized by dose response curves for 13 agonists and inhibition curves for six antagonists. alpha-Conotoxin MII did not inhibit this release, while alpha-conotoxin AuIB inhibited 50% of agonist-stimulated release. Comparison of this process with [(3)H]dopamine release from mouse striatal synaptosomes indicated that different forms of nicotinic acetylcholine receptors (nAChRs) may mediate these processes. This was confirmed by assays using mice homozygous for the beta 2 subunit null mutation. The deletion of the beta 2 subunit had no effect on agonist-stimulated acetylcholine release, but abolished agonist-stimulated release of dopamine from striatal synaptosomes. Mice heterozygous for the beta 2 subunit null mutation showed decreased dopamine release evoked by L-nicotine with no apparent change in EC(50) value, as well as similar decreases in both transient and persistent phases of release with no changes in desensitization rates.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0022-3042
pubmed:author
pubmed:issnType
Print
pubmed:volume
76
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
258-68
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:11145999-Acetylcholine, pubmed-meshheading:11145999-Alkaloids, pubmed-meshheading:11145999-Animals, pubmed-meshheading:11145999-Azocines, pubmed-meshheading:11145999-Calcium, pubmed-meshheading:11145999-Choline, pubmed-meshheading:11145999-Conotoxins, pubmed-meshheading:11145999-Corpus Striatum, pubmed-meshheading:11145999-Dopamine, pubmed-meshheading:11145999-Dose-Response Relationship, Drug, pubmed-meshheading:11145999-Female, pubmed-meshheading:11145999-Heterozygote, pubmed-meshheading:11145999-Homozygote, pubmed-meshheading:11145999-Male, pubmed-meshheading:11145999-Mesencephalon, pubmed-meshheading:11145999-Mice, pubmed-meshheading:11145999-Mice, Inbred C57BL, pubmed-meshheading:11145999-Mice, Mutant Strains, pubmed-meshheading:11145999-Nicotinic Agonists, pubmed-meshheading:11145999-Nicotinic Antagonists, pubmed-meshheading:11145999-Presynaptic Terminals, pubmed-meshheading:11145999-Protein Subunits, pubmed-meshheading:11145999-Quinolizines, pubmed-meshheading:11145999-Receptors, Nicotinic, pubmed-meshheading:11145999-Synaptosomes
pubmed:year
2001
pubmed:articleTitle
Nicotinic agonists stimulate acetylcholine release from mouse interpeduncular nucleus: a function mediated by a different nAChR than dopamine release from striatum.
pubmed:affiliation
Institute for Behavioral Genetics, University of Colorado, Boulder, Colorado 80309-0447, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't