|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2001-2-13
|
|
pubmed:abstractText |
Extracellular Nef which has been implicated in disease progression and development of AIDS induces IL-10, a potent immunosuppressive cytokine, in vitro. The present study was designed to examine whether the action of extracellular Nef is a protein tyrosine kinase (PTK)-dependent event. Anti-phosphotyrosine immunoblotting reveals that recombinant HIV-1 Nef induces rapid tyrosyl phosphorylation of several cellular proteins in human peripheral blood mononuclear cells. Pre-treatment of cells with herbimycin A, but not with genistein, significantly abolishes the Nef-induced tyrosine phosphorylation of cellular proteins. Furthermore, ELISA and RNase protection assays show that herbimycin A significantly blocks Nef-induced production of IL-10 at both the protein and the mRNA level. Genistein and aminogenistein have a much less blocking effect on the ability of Nef to induce IL-10. These results provide evidence for the involvement of a herbimycin A-sensitive PTK in the signal transduction pathway for exogenous HIV-1 Nef.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Benzoquinones,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, nef,
http://linkedlifedata.com/resource/pubmed/chemical/Genistein,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-10,
http://linkedlifedata.com/resource/pubmed/chemical/Lactams, Macrocyclic,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Quinones,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/herbimycin,
http://linkedlifedata.com/resource/pubmed/chemical/nef Gene Products, Human...
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jan
|
|
pubmed:issn |
0165-2478
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
1
|
|
pubmed:volume |
75
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
97-101
|
|
pubmed:dateRevised |
2009-11-19
|
|
pubmed:meshHeading |
pubmed-meshheading:11137132-Benzoquinones,
pubmed-meshheading:11137132-Cells, Cultured,
pubmed-meshheading:11137132-Enzyme Inhibitors,
pubmed-meshheading:11137132-Extracellular Space,
pubmed-meshheading:11137132-Gene Products, nef,
pubmed-meshheading:11137132-Genistein,
pubmed-meshheading:11137132-HIV-1,
pubmed-meshheading:11137132-Humans,
pubmed-meshheading:11137132-Interleukin-10,
pubmed-meshheading:11137132-Lactams, Macrocyclic,
pubmed-meshheading:11137132-Protein-Tyrosine Kinases,
pubmed-meshheading:11137132-Quinones,
pubmed-meshheading:11137132-RNA, Messenger,
pubmed-meshheading:11137132-Recombinant Proteins,
pubmed-meshheading:11137132-Signal Transduction,
pubmed-meshheading:11137132-nef Gene Products, Human Immunodeficiency Virus
|
|
pubmed:year |
2001
|
|
pubmed:articleTitle |
Involvement of a herbimycin A-sensitive protein tyrosine kinase in extracellular action of HIV-1 Nef.
|
|
pubmed:affiliation |
Department of Pediatrics, University of South Florida College of Medicine, All Children's Hospital, 801 Sixth Street South, St. Petersburg, FL 33701, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
