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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2001-1-10
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pubmed:abstractText |
Bacterial DNA and related synthetic immunostimulatory oligodeoxyribonucleotides (ISS-ODN) stimulate innate immunity. However, the molecular recognition mechanism that initiates signaling in response to bacterial DNA and ISS-ODN has not been identified. Herein, we demonstrate that administration of bacterial DNA and ISS-ODN to mice lacking the catalytic subunit of DNA-PK (DNA-PKcs) and in vitro stimulation of BMDM from these mice result in defective induction of IL-6 and IL-12. Further analysis using BMDM of IKKbeta(-/-) revealed that both DNA-PKcs and IKKbeta are essential for normal cytokine production in response to ISS-ODN or bacterial DNA. ISS-ODN and bacterial DNA activate DNA-PK, which in turn contributes to activation of IKK and NF-kappaB. These results reveal a novel role of DNA-PKcs in innate immune responses and a link between DNA repair and innate immunity.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Androstadienes,
http://linkedlifedata.com/resource/pubmed/chemical/Chuk protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Activated Protein Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/I-kappa B Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/Ikbkb protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Ikbke protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/wortmannin
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0092-8674
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pubmed:author |
pubmed-author:ChenD JDJ,
pubmed-author:ChenYY,
pubmed-author:ChiCC,
pubmed-author:DASAA,
pubmed-author:GonyJJ,
pubmed-author:KarinMM,
pubmed-author:LoisAA,
pubmed-author:MASS,
pubmed-author:OuyangHH,
pubmed-author:RaoNN,
pubmed-author:TakabayashiKK
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pubmed:issnType |
Print
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pubmed:day |
8
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pubmed:volume |
103
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
909-18
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:11136976-Androstadienes,
pubmed-meshheading:11136976-Animals,
pubmed-meshheading:11136976-Bone Marrow Cells,
pubmed-meshheading:11136976-DNA, Bacterial,
pubmed-meshheading:11136976-DNA Repair,
pubmed-meshheading:11136976-DNA-Activated Protein Kinase,
pubmed-meshheading:11136976-DNA-Binding Proteins,
pubmed-meshheading:11136976-Enzyme Inhibitors,
pubmed-meshheading:11136976-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:11136976-I-kappa B Kinase,
pubmed-meshheading:11136976-Immunity, Innate,
pubmed-meshheading:11136976-Interleukin-12,
pubmed-meshheading:11136976-Interleukin-6,
pubmed-meshheading:11136976-Macrophages,
pubmed-meshheading:11136976-Mice,
pubmed-meshheading:11136976-Mice, Inbred C57BL,
pubmed-meshheading:11136976-NF-kappa B,
pubmed-meshheading:11136976-Oligodeoxyribonucleotides,
pubmed-meshheading:11136976-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:11136976-Phosphorylation,
pubmed-meshheading:11136976-Protein-Serine-Threonine Kinases,
pubmed-meshheading:11136976-RNA, Messenger
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pubmed:year |
2000
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pubmed:articleTitle |
DNA-PKcs is required for activation of innate immunity by immunostimulatory DNA.
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pubmed:affiliation |
The Sam and Rose Stein Institute for Research on Aging, University of California, San Diego 9500 Gilman Drive 92093, La Jolla, CA, USA. wmchu@ucsd.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Retracted Publication,
Research Support, Non-U.S. Gov't
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