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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2001-1-4
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pubmed:abstractText |
Immature retrovirus particles contain radially arranged Gag polyproteins in which the N termini lie at the membrane and the C termini extend toward the particle's center. We related image features to the polyprotein domain structure by combining mutagenesis with cryoelectron microscopy and image analysis. The matrix (MA) domain appears as a thin layer tightly associated with the inner face of the viral membrane, separated from the capsid (CA) layer by a low-density region corresponding to its C terminus. Deletion of the entire p6 domain has no effect on the width or spacing of the density layers, suggesting that p6 is not ordered in immature human immunodeficiency virus type 1 (HIV-1). In vitro assembly of a recombinant Gag polyprotein containing only capsid (CA) and nucleocapsid (NC) domains results in the formation of nonenveloped spherical particles which display two layers with density matching that of the CA-NC portion of immature HIV-1 Gag particles. Authentic, immature HIV-1 displays additional surface features and an increased density between the lipid bilayers which reflect the presence of gp41. The other internal features match those of virus-like particles.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11134289-10208938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11134289-10322217,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11134289-9499062,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11134289-9971814
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
75
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
759-71
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11134289-Capsid,
pubmed-meshheading:11134289-Cell Membrane,
pubmed-meshheading:11134289-Cryoelectron Microscopy,
pubmed-meshheading:11134289-Gene Deletion,
pubmed-meshheading:11134289-Gene Products, gag,
pubmed-meshheading:11134289-HIV-1,
pubmed-meshheading:11134289-Humans,
pubmed-meshheading:11134289-Image Processing, Computer-Assisted,
pubmed-meshheading:11134289-Lipid Bilayers,
pubmed-meshheading:11134289-Nucleocapsid,
pubmed-meshheading:11134289-Protein Structure, Tertiary,
pubmed-meshheading:11134289-Recombinant Proteins,
pubmed-meshheading:11134289-Viral Matrix Proteins,
pubmed-meshheading:11134289-Virion,
pubmed-meshheading:11134289-Virus Assembly,
pubmed-meshheading:11134289-gag Gene Products, Human Immunodeficiency Virus
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pubmed:year |
2001
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pubmed:articleTitle |
Organization of immature human immunodeficiency virus type 1.
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pubmed:affiliation |
The Structural Biology Programme, European Molecular Biology Laboratory, D69012 Heidelberg, Federal Republic of Germany.
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pubmed:publicationType |
Journal Article
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