Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2001-2-15
pubmed:abstractText
Cytoplasmic dynein is a multisubunit protein complex responsible for the intracellular movement of membranous organelles and other cargo along microtubules. The heavy chains contain the motor domains, while the intermediate chain and other subunits are important for binding to cargo. There are at least five different intermediate chain polypeptides, the products of alternative splicing of two genes. The cytoplasmic dynein intermediate chains are also phosphorylated. The expression of the different intermediate chain mRNAs is characterized by reverse transcription-polymerase chain reactions using oligonucleotide primers appropriate for the alternative splicing sites. The presence of the different intermediate chain polypeptide isoforms is determined by two-dimensional gel analysis of cytoplasmic dynein samples. The phosphorylation state of the polypeptides is determined by treatment of immunoprecipitated cytoplasmic dynein with protein phosphatase and analysis of changes in polypeptide spot distribution after two-dimensional gel electrophoresis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1046-2023
pubmed:author
pubmed:copyrightInfo
Copyright 2000 Academic Press.
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
307-16
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Biochemical and molecular analysis of the mammalian cytoplasmic dynein intermediate chain.
pubmed:affiliation
Department of Cell Biology, School of Medicine, Charlottesville, Virginia 22908, USA.
pubmed:publicationType
Journal Article