| pubmed-article:11132771 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11132771 | lifeskim:mentions | umls-concept:C0030705 | lld:lifeskim |
| pubmed-article:11132771 | lifeskim:mentions | umls-concept:C0004096 | lld:lifeskim |
| pubmed-article:11132771 | lifeskim:mentions | umls-concept:C0038056 | lld:lifeskim |
| pubmed-article:11132771 | lifeskim:mentions | umls-concept:C0806987 | lld:lifeskim |
| pubmed-article:11132771 | lifeskim:mentions | umls-concept:C0205178 | lld:lifeskim |
| pubmed-article:11132771 | lifeskim:mentions | umls-concept:C1155266 | lld:lifeskim |
| pubmed-article:11132771 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:11132771 | pubmed:issue | 3-4 | lld:pubmed |
| pubmed-article:11132771 | pubmed:dateCreated | 2000-12-29 | lld:pubmed |
| pubmed-article:11132771 | pubmed:abstractText | Examination of sputum provides a direct method to investigate airway inflammation non-invasively in particular Th1 (IL-2, IFN-gamma) and Th2 (IL-4, IL-10) cytokine production. IL-2, IL-4, IL-10 and IFN-gamma cytokine were studied in induced sputum mononuclear cells of asthmatic patients. Sputum induction was performed on 10 patients and 10 normal controls. Basal and mitogen-stimulated cytokine production was determined in induced sputum T-cell culture. Supernatants were collected and assayed not only with specific ELISA but also with polymerase chain reaction (PCR) techniques. Data showed a significantly higher production of IL-10 by both the ELISA and the RT-PCR techniques in asthmatic patients compared with sputum mononuclear cells from healthy controls. IL-4 production was detected at a low level using the ELISA method in asthmatic patients. The RT-PCR analysis detected a significantly IL-4-mRNA expression in all asthmatic patients, compared with controls. Results of IL-10 and IL-4 mRNA expression were reproducible. We did not find any alteration in the expression of the type 1 derived cytokines (IL-2 and IFN-gamma) in asthmatic patients or in healthy controls. Our study showed a tendency of induced sputum mononuclear cells to express a Th2-like cytokine pattern in acute exacerbation of asthmatic patients, where IL-10 and IL-4 are synthesized in larger amounts. The combination of sputum induction as a non-invasive tool to explore the lung and the identification of disease-associated cytokine expression and of specific cytokine mRNA should help elucidate mechanisms of the immunologically mediated inflammatory responses in asthma. | lld:pubmed |
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| pubmed-article:11132771 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11132771 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11132771 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:11132771 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11132771 | pubmed:issn | 0962-9351 | lld:pubmed |
| pubmed-article:11132771 | pubmed:author | pubmed-author:AyedKK | lld:pubmed |
| pubmed-article:11132771 | pubmed:author | pubmed-author:BrahimM BMB | lld:pubmed |
| pubmed-article:11132771 | pubmed:author | pubmed-author:HamzaouiKK | lld:pubmed |
| pubmed-article:11132771 | pubmed:author | pubmed-author:HamzaouiAA | lld:pubmed |
| pubmed-article:11132771 | pubmed:author | pubmed-author:ZhiouaAA | lld:pubmed |
| pubmed-article:11132771 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11132771 | pubmed:volume | 9 | lld:pubmed |
| pubmed-article:11132771 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11132771 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11132771 | pubmed:pagination | 147-53 | lld:pubmed |
| pubmed-article:11132771 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:11132771 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:11132771 | pubmed:articleTitle | Inflammatory response in induced sputum mononuclear cells from patients with acute exacerbation of asthma. | lld:pubmed |
| pubmed-article:11132771 | pubmed:affiliation | A. Mami Hospital, Department of Pediatric Respiratory Diseases, Ariana. Kamel.Hamzaoui@fmt.rnu.tn | lld:pubmed |
| pubmed-article:11132771 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11132771 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11132771 | lld:pubmed |
