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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3-4
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pubmed:dateCreated |
2000-12-29
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pubmed:abstractText |
Examination of sputum provides a direct method to investigate airway inflammation non-invasively in particular Th1 (IL-2, IFN-gamma) and Th2 (IL-4, IL-10) cytokine production. IL-2, IL-4, IL-10 and IFN-gamma cytokine were studied in induced sputum mononuclear cells of asthmatic patients. Sputum induction was performed on 10 patients and 10 normal controls. Basal and mitogen-stimulated cytokine production was determined in induced sputum T-cell culture. Supernatants were collected and assayed not only with specific ELISA but also with polymerase chain reaction (PCR) techniques. Data showed a significantly higher production of IL-10 by both the ELISA and the RT-PCR techniques in asthmatic patients compared with sputum mononuclear cells from healthy controls. IL-4 production was detected at a low level using the ELISA method in asthmatic patients. The RT-PCR analysis detected a significantly IL-4-mRNA expression in all asthmatic patients, compared with controls. Results of IL-10 and IL-4 mRNA expression were reproducible. We did not find any alteration in the expression of the type 1 derived cytokines (IL-2 and IFN-gamma) in asthmatic patients or in healthy controls. Our study showed a tendency of induced sputum mononuclear cells to express a Th2-like cytokine pattern in acute exacerbation of asthmatic patients, where IL-10 and IL-4 are synthesized in larger amounts. The combination of sputum induction as a non-invasive tool to explore the lung and the identification of disease-associated cytokine expression and of specific cytokine mRNA should help elucidate mechanisms of the immunologically mediated inflammatory responses in asthma.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11132771-10727445,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11132771-10844523,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11132771-1361126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11132771-1371884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11132771-1530827,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11132771-1735191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11132771-1825981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11132771-1972164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11132771-2531194,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11132771-2836253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11132771-7648458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11132771-7869046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11132771-7980676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11132771-8011301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11132771-8132221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11132771-8245792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11132771-8386517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11132771-8564094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11132771-8811054
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0962-9351
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
147-53
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11132771-Adult,
pubmed-meshheading:11132771-Asthma,
pubmed-meshheading:11132771-Base Sequence,
pubmed-meshheading:11132771-Case-Control Studies,
pubmed-meshheading:11132771-Cytokines,
pubmed-meshheading:11132771-DNA Primers,
pubmed-meshheading:11132771-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:11132771-Humans,
pubmed-meshheading:11132771-Inflammation Mediators,
pubmed-meshheading:11132771-Interferon-gamma,
pubmed-meshheading:11132771-Interleukin-10,
pubmed-meshheading:11132771-Interleukin-2,
pubmed-meshheading:11132771-Interleukin-4,
pubmed-meshheading:11132771-RNA, Messenger,
pubmed-meshheading:11132771-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11132771-Sputum,
pubmed-meshheading:11132771-Th1 Cells,
pubmed-meshheading:11132771-Th2 Cells
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pubmed:year |
2000
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pubmed:articleTitle |
Inflammatory response in induced sputum mononuclear cells from patients with acute exacerbation of asthma.
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pubmed:affiliation |
A. Mami Hospital, Department of Pediatric Respiratory Diseases, Ariana. Kamel.Hamzaoui@fmt.rnu.tn
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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