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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0017262,
umls-concept:C0033414,
umls-concept:C0039341,
umls-concept:C0086418,
umls-concept:C0162825,
umls-concept:C0185117,
umls-concept:C0600210,
umls-concept:C1334043,
umls-concept:C1413189,
umls-concept:C2911684
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pubmed:issue |
12
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pubmed:dateCreated |
2000-12-21
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pubmed:abstractText |
Human basophils and mast cells express the chemokine receptor CCR3, which binds the chemokines eotaxin and RANTES. HIV-1 Tat protein is a potent chemoattractant for basophils and lung mast cells obtained from healthy individuals seronegative for Abs to HIV-1 and HIV-2. Tat protein induced a rapid and transient Ca(2+) influx in basophils and mast cells, analogous to beta-chemokines. Tat protein neither induced histamine release from human basophils and mast cells nor increased IL-3-stimulated histamine secretion from basophils. The chemotactic activity of Tat protein was blocked by preincubation of FcepsilonRI(+) cells with anti-CCR3 Ab. Preincubation of Tat with a mAb anti-Tat (aa 1-86) blocked the migration induced by Tat. In contrast, a mAb specific for the basic region (aa 46-60) did not inhibit the chemotactic effect of Tat protein. Tat protein or eotaxin desensitized basophils to a subsequent challenge with the autologous or the heterologous stimulus. Preincubation of basophils with Tat protein up-regulated the level of CCR3 mRNA and the surface expression of the CCR3 receptor. Tat protein is the first identified HIV-1-encoded beta-chemokine homologue that influences the directional migration of human FcepsilonRI(+) cells and the expression of surface receptor CCR3 on these cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/CCL11 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/CCR3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL11,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines, CC,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, tat,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, CCR3,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Chemokine,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, HIV,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, IgE,
http://linkedlifedata.com/resource/pubmed/chemical/tat Gene Products, Human...
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
165
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7171-9
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:11120849-Adult,
pubmed-meshheading:11120849-Antibodies, Monoclonal,
pubmed-meshheading:11120849-Basophils,
pubmed-meshheading:11120849-Calcium,
pubmed-meshheading:11120849-Calcium Signaling,
pubmed-meshheading:11120849-Cell Migration Inhibition,
pubmed-meshheading:11120849-Cell Movement,
pubmed-meshheading:11120849-Chemokine CCL11,
pubmed-meshheading:11120849-Chemokines, CC,
pubmed-meshheading:11120849-Chemotaxis, Leukocyte,
pubmed-meshheading:11120849-Cytokines,
pubmed-meshheading:11120849-Epitopes,
pubmed-meshheading:11120849-Gene Expression Regulation,
pubmed-meshheading:11120849-Gene Products, tat,
pubmed-meshheading:11120849-HIV-1,
pubmed-meshheading:11120849-Histamine Release,
pubmed-meshheading:11120849-Humans,
pubmed-meshheading:11120849-Lung,
pubmed-meshheading:11120849-Mast Cells,
pubmed-meshheading:11120849-RNA, Messenger,
pubmed-meshheading:11120849-Receptors, CCR3,
pubmed-meshheading:11120849-Receptors, Chemokine,
pubmed-meshheading:11120849-Receptors, HIV,
pubmed-meshheading:11120849-Receptors, IgE,
pubmed-meshheading:11120849-Sequence Homology, Amino Acid,
pubmed-meshheading:11120849-Up-Regulation,
pubmed-meshheading:11120849-tat Gene Products, Human Immunodeficiency Virus
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pubmed:year |
2000
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pubmed:articleTitle |
Tat protein is an HIV-1-encoded beta-chemokine homolog that promotes migration and up-regulates CCR3 expression on human Fc epsilon RI+ cells.
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pubmed:affiliation |
Division of Clinical Immunology and Allergy, University of Naples Federico II, Naples, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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