Linked Life Data

11112152

Source:http://linkedlifedata.com/resource/pubmed/id/11112152

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2001-1-24
pubmed:abstractText
Several studies suggest that anesthetics modulate the immune response. The aim of this study was to investigate the effect of halothane and thiopental on the lung inflammatory response. Rats submitted or not to intratracheal (IT) instillation of lipopolysaccharides (LPS) were anesthetized with either halothane (0. 5, 1, or 1.5%) or thiopental (60 mg. kg(-1)) and mechanically ventilated for 4 h. Control rats were treated or not by LPS without anesthesia. Lung inflammation was assessed by total and differential cell counts in bronchoalveolar lavage fluids (BALF) and by cytokine measurements (tumor necrosis factor-alpha [TNF-alpha], interleukin-6 [IL-6], macrophage inflammatory protein-2 [MIP-2], and monocyte chemoattractant protein-1 [MCP-1]) in BALF and lung homogenates. In the absence of LPS treatment, neither halothane nor thiopental modified the moderate inflammatory response induced by tracheotomy or mechanical ventilation. Cell recruitment and cytokine concentrations were increased in all groups receiving IT LPS. However, in halothane-anesthetized rats (halothane > or = 1%), but not in thiopental-anesthetized rats, the LPS-induced lung inflammation was altered in a dose-dependent manner. Indeed, when using 1% halothane, polymorphonuclear leukocyte (PMN) recruitment was decreased by 55% (p < 0.001) and TNF-alpha, IL-6, and MIP-2 concentrations in BALF and lung homogenates were decreased by more than 60% (p < 0.001) whereas total protein and MCP-1 concentrations remained unchanged. The decrease of MIP-2 (observed at the protein and messenger RNA [mRNA] level) was strongly correlated to the decrease of PMN recruitment (r = 0.73, p < 0.05). This halothane-reduced lung inflammatory response was transient and was reversed 20 h after the end of the anesthesia. Our study shows that halothane > or = 1%, delivered during 4 h by mechanical ventilation, but not mechanical ventilation per se, alters the early LPS-induced lung inflammation in the rat, suggesting a specific effect of halothane on this response.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1073-449X
pubmed:author
pubmed:issnType
Print
pubmed:volume
162
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2278-86
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11112152-Anesthetics, Inhalation, pubmed-meshheading:11112152-Anesthetics, Intravenous, pubmed-meshheading:11112152-Animals, pubmed-meshheading:11112152-Base Sequence, pubmed-meshheading:11112152-Bronchoalveolar Lavage Fluid, pubmed-meshheading:11112152-Escherichia coli, pubmed-meshheading:11112152-Halothane, pubmed-meshheading:11112152-Lipopolysaccharides, pubmed-meshheading:11112152-Lung, pubmed-meshheading:11112152-Male, pubmed-meshheading:11112152-Molecular Sequence Data, pubmed-meshheading:11112152-Pneumonia, pubmed-meshheading:11112152-Polymerase Chain Reaction, pubmed-meshheading:11112152-Rats, pubmed-meshheading:11112152-Rats, Sprague-Dawley, pubmed-meshheading:11112152-Respiration, Artificial, pubmed-meshheading:11112152-Specific Pathogen-Free Organisms, pubmed-meshheading:11112152-Thiopental, pubmed-meshheading:11112152-Time Factors
pubmed:year
2000
pubmed:articleTitle
Halothane reduces the early lipopolysaccharide-induced lung inflammation in mechanically ventilated rats.
pubmed:affiliation
Unité INSERM 408, Faculté de Médecine Xavier Bichat, Paris, France.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't