Linked Life Data

11108972

Source:http://linkedlifedata.com/resource/pubmed/id/11108972

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-3
pubmed:dateCreated
2001-1-11
pubmed:abstractText
Commonly used methods for microdialysis recovery measurement are reviewed and the zero flow and no net flux methods are suggested as the most robust in practice. Six different mathematical models of microdialysis assumptions are investigated and compared for varying dialysis probe radius. One transmitter (dopamine), three metabolites (DOPAC, HVA and 5HIAA) and two drugs (caffeine and theophylline) were studied. Histology and functional response to a drug were measured. Deficiencies were demonstrated for several of the models, the one best explaining experimental data includes both passive diffusion and active tissue regulation in a cylindrical symmetric geometry. The recovery decreased with decreasing probe radius but smaller probes caused less tissue injury. It is concluded that a mathematical model of microdialysis must include diffusional and physiological processes in order to accurately account for experimentally observed phenomena. The experiments also demonstrated that, for small brain nuclei, the size of the nucleus may influence the recovery.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0169-409X
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
45
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
149-67
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
On mathematical models of microdialysis: geometry, steady-state models, recovery and probe radius.
pubmed:affiliation
Department of Clinical Pharmacology, Karolinska Institute, Huddinge Hospital, SE-14186, Huddinge, Sweden. lars.stahle@pharmlab.hs.sll.se
pubmed:publicationType
Journal Article