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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2001-2-28
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pubmed:abstractText |
The minimum element from the Drosophila period promoter capable of driving in vivo cycling mRNA is the 69 bp circadian regulatory sequence (CRS). In cell culture, an 18 bp E-box element from the period promoter is regulated by five genes that are involved in the regulation of circadian expression in flies. This E-box is a target for transcriptional activation by bHLH-PAS proteins dCLOCK (dCLK) and CYCLE (CYC), this activation is inhibited by PERIOD (PER) and TIMELESS (TIM) together, and inhibition of dCLK/CYC by PER and TIM is blocked by CRYPTOCHROME (CRY) in the presence of light. Here, the same 18 bp E-box region generated rhythmic expression of luciferase in flies under both light-dark cycling and constant conditions. Flies heterozygous for the Clke(jrk) mutation maintained rhythmic expression from the E-box although at a lower level than wild type. Homozygous mutant Clk(jrk) animals had drastically lowered and arrhythmic expression. In a per01 background, expression from the E-box was high and not rhythmic. Transcription mediated by the per E-box was restricted to the same spatial pattern as the CRS. The per E-box DNA element and cognate binding proteins can confer per-like temporal and spatial expression. This demonstrates in vivo that the known circadian genes that form the core of the circadian oscillator in Drosophila integrate their activities at a single DNA element.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0748-7304
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
462-71
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:11106063-Animals,
pubmed-meshheading:11106063-Animals, Genetically Modified,
pubmed-meshheading:11106063-Basic Helix-Loop-Helix Transcription Factors,
pubmed-meshheading:11106063-Circadian Rhythm,
pubmed-meshheading:11106063-Drosophila,
pubmed-meshheading:11106063-Drosophila Proteins,
pubmed-meshheading:11106063-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:11106063-Helix-Loop-Helix Motifs,
pubmed-meshheading:11106063-Luciferases,
pubmed-meshheading:11106063-Luminescent Measurements,
pubmed-meshheading:11106063-Male,
pubmed-meshheading:11106063-Nuclear Proteins,
pubmed-meshheading:11106063-Period Circadian Proteins,
pubmed-meshheading:11106063-Promoter Regions, Genetic,
pubmed-meshheading:11106063-Trans-Activators,
pubmed-meshheading:11106063-Transcription, Genetic,
pubmed-meshheading:11106063-Transcriptional Activation,
pubmed-meshheading:11106063-beta-Galactosidase
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pubmed:year |
2000
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pubmed:articleTitle |
The period E-box is sufficient to drive circadian oscillation of transcription in vivo.
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pubmed:affiliation |
Department of Cell Biology and NSF Center for Biological Timing, The Scripps Research Institute, La Jolla, CA 92037, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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