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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2001-5-23
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pubmed:abstractText |
The angiogenic effects of vascular endothelial growth factor are mediated predominantly by the FLK-1/KDR receptor. An understanding of the transcriptional control mechanisms underlying flk-1/KDR expression should provide insight into the molecular basis of angiogenesis. In this study, we show that transforming growth factor-beta(1) (TGF-beta(1)) down-regulates expression of the endogenous flk-1/KDR gene in endothelial cells. In transient transfection assays, this effect was mapped to a palindromic GATA site in the 5'-untranslated region. In electrophoretic mobility shift assays, the palindromic GATA site was shown to bind to two molecules of GATA protein. Moreover, DNA-GATA interactions were inhibited by TGF-beta(1). Finally, in cotransfection assays, transactivation of the flk-1/KDR promoter by GATA-1 or GATA-2 was attenuated in TGF-beta(1)-treated cells. Taken together, these results suggest that the TGF-beta-1-mediated inhibition of the flk-1/KDR gene is mediated by a 5'-untranslated region palindromic GATA site.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/5' Untranslated Regions,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/GATA2 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/GATA2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Vascular Endothelial...,
http://linkedlifedata.com/resource/pubmed/chemical/TGFB1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta1
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
16
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pubmed:volume |
276
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5395-402
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:11098056-5' Untranslated Regions,
pubmed-meshheading:11098056-Animals,
pubmed-meshheading:11098056-Cattle,
pubmed-meshheading:11098056-Cell Line,
pubmed-meshheading:11098056-Cells, Cultured,
pubmed-meshheading:11098056-Consensus Sequence,
pubmed-meshheading:11098056-DNA,
pubmed-meshheading:11098056-DNA Footprinting,
pubmed-meshheading:11098056-DNA-Binding Proteins,
pubmed-meshheading:11098056-Down-Regulation,
pubmed-meshheading:11098056-Endothelium, Vascular,
pubmed-meshheading:11098056-GATA2 Transcription Factor,
pubmed-meshheading:11098056-Humans,
pubmed-meshheading:11098056-Promoter Regions, Genetic,
pubmed-meshheading:11098056-RNA, Messenger,
pubmed-meshheading:11098056-Receptor Protein-Tyrosine Kinases,
pubmed-meshheading:11098056-Receptors, Growth Factor,
pubmed-meshheading:11098056-Receptors, Vascular Endothelial Growth Factor,
pubmed-meshheading:11098056-Transcription Factors,
pubmed-meshheading:11098056-Transcriptional Activation,
pubmed-meshheading:11098056-Transforming Growth Factor beta,
pubmed-meshheading:11098056-Transforming Growth Factor beta1
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pubmed:year |
2001
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pubmed:articleTitle |
Transforming growth factor-beta 1-mediated inhibition of the flk-1/KDR gene is mediated by a 5'-untranslated region palindromic GATA site.
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pubmed:affiliation |
Department of Molecular Medicine, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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