Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2000-12-20
pubmed:abstractText
1. The NO-dependent component of cyclic AMP-induced vasorelaxation in rat pulmonary arteries is critically dependent on extracellular L-arginine but independent of endothelial cell intracellular [Ca(2+)]. We examined whether L-arginine uptake was also essential for NO production induced by passive stretch or isometric tension, processes also reported to be Ca(2+)-independent. 2. The passive length-tension curve was depressed by physiological concentrations of L-arginine (400 microM; P<0.05). Inhibition of the y(+) transporter with 10 mM L-lysine, NO synthase with L-NAME (100 microM), or protein tyrosine kinase with erbstatin A (30 microM) caused identical upward shifts (P<0.001), alone or in combination. Tyrphostin 23 was similar to erbstatin A, whilst the inactive analogue tyrphostin A1 and genistein were without effect. 3. L-arginine (400 microM) shifted the PGF(2 alpha) concentration-response curve under isometric conditions to the right (P<0.05), whereas L-NAME or L-lysine caused a leftward shift (P<0.001). Tyrphostin 23 (30 microM) more than reversed the L-arginine-induced suppression of PGF(2 alpha)-induced tension; subsequent addition of L-NAME had no effect. The L-lysine-sensitive component of CPT cyclic AMP-induced vasorelaxation was abolished by erbstatin A. 4. ACh-induced vasorelaxation was approximately 80% inhibited by L-NAME, but was not affected by L-lysine or 400 microM L-arginine. Erbstatin A reduced the vasorelaxation by only approximately 25%. 5. We conclude that activation of NO production by stretch, isometric tension, or cyclic AMP in rat pulmonary arteries is critically dependent on the presence and uptake of physiological concentrations of extracellular L-arginine, and protein tyrosine kinase activity. This directly contrasts with ACh-induced vasorelaxation, which was independent of extracellular L-arginine, and relatively unaffected by tyrosine kinase inhibition.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11090123-10069529, http://linkedlifedata.com/resource/pubmed/commentcorrection/11090123-10077589, http://linkedlifedata.com/resource/pubmed/commentcorrection/11090123-10342824, http://linkedlifedata.com/resource/pubmed/commentcorrection/11090123-10369485, http://linkedlifedata.com/resource/pubmed/commentcorrection/11090123-10372832, http://linkedlifedata.com/resource/pubmed/commentcorrection/11090123-10662891, http://linkedlifedata.com/resource/pubmed/commentcorrection/11090123-10671564, http://linkedlifedata.com/resource/pubmed/commentcorrection/11090123-10725285, http://linkedlifedata.com/resource/pubmed/commentcorrection/11090123-10882383, http://linkedlifedata.com/resource/pubmed/commentcorrection/11090123-10912464, http://linkedlifedata.com/resource/pubmed/commentcorrection/11090123-1401062, http://linkedlifedata.com/resource/pubmed/commentcorrection/11090123-1514644, http://linkedlifedata.com/resource/pubmed/commentcorrection/11090123-1659406, http://linkedlifedata.com/resource/pubmed/commentcorrection/11090123-1720542, http://linkedlifedata.com/resource/pubmed/commentcorrection/11090123-1852778, http://linkedlifedata.com/resource/pubmed/commentcorrection/11090123-2268354, http://linkedlifedata.com/resource/pubmed/commentcorrection/11090123-2594944, http://linkedlifedata.com/resource/pubmed/commentcorrection/11090123-2912454, http://linkedlifedata.com/resource/pubmed/commentcorrection/11090123-7491975, http://linkedlifedata.com/resource/pubmed/commentcorrection/11090123-8274622, http://linkedlifedata.com/resource/pubmed/commentcorrection/11090123-8620594, http://linkedlifedata.com/resource/pubmed/commentcorrection/11090123-8922759, http://linkedlifedata.com/resource/pubmed/commentcorrection/11090123-9038906, http://linkedlifedata.com/resource/pubmed/commentcorrection/11090123-9395443, http://linkedlifedata.com/resource/pubmed/commentcorrection/11090123-9452418, http://linkedlifedata.com/resource/pubmed/commentcorrection/11090123-9466952, http://linkedlifedata.com/resource/pubmed/commentcorrection/11090123-9546377, http://linkedlifedata.com/resource/pubmed/commentcorrection/11090123-9712842, http://linkedlifedata.com/resource/pubmed/commentcorrection/11090123-9765266, http://linkedlifedata.com/resource/pubmed/commentcorrection/11090123-9927704
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/8-((4-chlorophenyl)thio)cyclic-3',5'..., http://linkedlifedata.com/resource/pubmed/chemical/Acetylcholine, http://linkedlifedata.com/resource/pubmed/chemical/Arginine, http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP, http://linkedlifedata.com/resource/pubmed/chemical/Dinoprost, http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/Hydroquinones, http://linkedlifedata.com/resource/pubmed/chemical/Lysine, http://linkedlifedata.com/resource/pubmed/chemical/NG-Nitroarginine Methyl Ester, http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide, http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Thionucleotides, http://linkedlifedata.com/resource/pubmed/chemical/Tyrphostins, http://linkedlifedata.com/resource/pubmed/chemical/Vasodilator Agents, http://linkedlifedata.com/resource/pubmed/chemical/erbstatin
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0007-1188
pubmed:author
pubmed:issnType
Print
pubmed:volume
131
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1475-81
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:11090123-Acetylcholine, pubmed-meshheading:11090123-Animals, pubmed-meshheading:11090123-Arginine, pubmed-meshheading:11090123-Cyclic AMP, pubmed-meshheading:11090123-Dinoprost, pubmed-meshheading:11090123-Dose-Response Relationship, Drug, pubmed-meshheading:11090123-Enzyme Inhibitors, pubmed-meshheading:11090123-Hydroquinones, pubmed-meshheading:11090123-Isometric Contraction, pubmed-meshheading:11090123-Lysine, pubmed-meshheading:11090123-Male, pubmed-meshheading:11090123-NG-Nitroarginine Methyl Ester, pubmed-meshheading:11090123-Nitric Oxide, pubmed-meshheading:11090123-Protein-Tyrosine Kinases, pubmed-meshheading:11090123-Pulmonary Artery, pubmed-meshheading:11090123-Rats, pubmed-meshheading:11090123-Rats, Wistar, pubmed-meshheading:11090123-Stress, Mechanical, pubmed-meshheading:11090123-Thionucleotides, pubmed-meshheading:11090123-Tyrphostins, pubmed-meshheading:11090123-Vasodilation, pubmed-meshheading:11090123-Vasodilator Agents
pubmed:year
2000
pubmed:articleTitle
Essential role of L-arginine uptake and protein tyrosine kinase activity for NO-dependent vasorelaxation induced by stretch, isometric tension and cyclic AMP in rat pulmonary arteries.
pubmed:affiliation
Department of Respiratory Medicine and Allergy, Guy's, King's and St Thomas' School of Medicine, King's College London, Guy's Campus, London SE1 9RT.
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