Linked Life Data

11080182

Source:http://linkedlifedata.com/resource/pubmed/id/11080182

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2000-11-20
pubmed:abstractText
Microglial activation has recently been recognized as a cause of damage in various neurodegenerative diseases. A possible mechanism underlying this damage is the activation of microglia by serum factors leaked through a disruption of the blood-brain barrier, which in turn trigger microglial cell proliferation and the release of various substances toxic to neurons, such as superoxide (O(2)(-)). We recently reported that serum albumin enhanced O(2)(-) production in cultured rat microglia stimulated by phorbol ester. In the present report, we identify the active site of this enhancement within the albumin molecule. We purified an active subfragment from trypsin-treated bovine serum albumin that was composed of 12-mer and 33-mer peptides connected by a disulfide bond. The chemically synthesized 12-mer peptide showed activity within a concentration range ( approximately 10(-7) M:) equivalent to that of albumin. The activities of a series of synthesized peptides conclusively indicated that the minimum active sequence was Leu-His-Thr-Leu. The present study may shed light on the mechanism of neuronal cell damage in various neurodegenerative diseases.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0022-3042
pubmed:author
pubmed:issnType
Print
pubmed:volume
75
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2309-15
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Identification of a peptide sequence in albumin that potentiates superoxide production by microglia.
pubmed:affiliation
Department of Physiology, Ehime University School of Medicine, Ehime, Japan. yoichi@dbs.p.kanazawa-u.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't