Linked Life Data

11079554

Source:http://linkedlifedata.com/resource/pubmed/id/11079554

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
16
pubmed:dateCreated
2001-2-12
pubmed:abstractText
Protein expression profiling by proteomics has the potential to be an ideal tool for the description of changes in complex biological systems or in the characterization of a disease. This study analyzes in more quantitative terms how methodological drawbacks of the current technology can hamper the comprehensive analysis of large sets of spot patterns by statistical means. Irreproducibilities in spot intensities due to silver staining and geometric distortions of the spot patterns inherent to the electrophoresis procedure push even the semiautomatic alignment and matching to their limits. This leads to reduced matching efficiencies for identical spots if no additional exhaustive manual matching is performed for every single spot in all gels. As a consequence, only a limited number of spots can be matched in all gels of a large set. The statistical pattern analysis of such data sets will thus not allow the comprehensive description of relevant pattern changes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0173-0835
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3345-50
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Observations on the reproducibility and matching efficiency of two-dimensional electrophoresis gels: consequences for comprehensive data analysis.
pubmed:affiliation
Boehringer-Ingelheim, Vienna, Austria. tilman.voss@vie.boehringer-ingelheim.com
pubmed:publicationType
Journal Article