|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
23
|
|
pubmed:dateCreated |
2000-12-4
|
|
pubmed:abstractText |
A molecular epidemiology study was conducted among more than 100 human immunodeficiency virus type 1 (HIV-1) subtype C seropositive intravenous drug users (IDUs) from China. Genotyping based on the envelope C2V3 coding region revealed the highest homology of the most prevalent virus strains circulating throughout China to subtype C sequences of Indian origin. Based on these results, a virtually full-length genome representing the most prevalent class of clade C strains circulating throughout China was directly amplified from peripheral blood mononuclear cells of a selected HIV-infected IDU and subcloned. Sequence analysis identified a mosaic structure, suggesting extensive intersubtype recombination events between genomes of the prevalent clade C and (B')-subtype Thai virus strains of that geographic region. Recombinant Identification Program analysis and phylogenetic bootstrapping suggested that there were 10 breakpoints (i) in the gag-pol coding region, (ii) in vpr and at the 3' end of the vpu gene, and (iii) in the nef open reading frame. (B')-sequences therefore include (i) several insertions in the gag-pol coding region; (ii) 3'-vpr, the complete vpu gene, and the first exons of tat and rev; and (iii) the 5' half of the nef gene. Breakpoints located in the vpr/vpu coding region as well as in the nef gene of 97cn54 were found at almost identical positions of all subtype C strains isolated from IDUs living in different areas of China, suggesting a common ancestor for the C/B' recombinant strains. More than 50% of well-defined subtype B-derived cytotoxic T-lymphocyte epitopes within Gag and Pol and 10% of the known epitopes in Env were found to exactly match sequences within in this clade C/B' chimeric reference strain. These results may substantially facilitate a biological comparison of clade C-derived reference strains as well as the generation of useful reagents supporting vaccine-related efforts in China.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11070037-10390833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11070037-10419458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11070037-10470067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11070037-10566148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11070037-11070028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11070037-1377403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11070037-1501299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11070037-1713810,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11070037-2014240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11070037-7265238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11070037-7483282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11070037-7545977,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11070037-7566026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11070037-7637010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11070037-7666516,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11070037-7707498,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11070037-7857556,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11070037-8035531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11070037-8230445,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11070037-8255784,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11070037-8573403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11070037-8586027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11070037-8709215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11070037-8726508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11070037-8748018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11070037-8794346,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11070037-8948379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11070037-8957675,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11070037-9491920,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11070037-9621027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11070037-9632380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11070037-9683568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11070037-9759480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11070037-9812517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11070037-9847317
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Dec
|
|
pubmed:issn |
0022-538X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
74
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
11367-76
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:11070037-Amino Acid Sequence,
pubmed-meshheading:11070037-China,
pubmed-meshheading:11070037-Cloning, Molecular,
pubmed-meshheading:11070037-Epitopes, T-Lymphocyte,
pubmed-meshheading:11070037-Genome, Viral,
pubmed-meshheading:11070037-HIV-1,
pubmed-meshheading:11070037-Humans,
pubmed-meshheading:11070037-Molecular Sequence Data,
pubmed-meshheading:11070037-Phylogeny,
pubmed-meshheading:11070037-Recombination, Genetic,
pubmed-meshheading:11070037-Substance Abuse, Intravenous,
pubmed-meshheading:11070037-T-Lymphocytes, Cytotoxic
|
|
pubmed:year |
2000
|
|
pubmed:articleTitle |
Characterization of a virtually full-length human immunodeficiency virus type 1 genome of a prevalent intersubtype (C/B') recombinant strain in China.
|
|
pubmed:affiliation |
Institute of Medical Microbiology, University of Regensburg, D-93053 Regensburg, Germany.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
