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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2000-12-11
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pubmed:abstractText |
The Notch ligand, Jagged-1, plays an essential role in tissue formation during embryonic development of primitive organisms. However, little is known regarding the role of Jagged-1 in the regulation of tissue-specific stem cells or its function in humans. Here, we show that uncommitted human hematopoietic cells and cells that comprise the putative blood stem cell microenvironment express Jagged-1 and the Notch receptors. Addition of a soluble form of human Jagged-1 to cultures of purified primitive human blood cells had modest effects in augmenting cytokine-induced proliferation of progenitors. However, intravenous transplantation of cultured cells into immunodeficient mice revealed that human (h)Jagged-1 induces the survival and expansion of human stem cells capable of pluripotent repopulating capacity. Our findings demonstrate that hJagged-1 represents a novel growth factor of human stem cells, thereby providing an opportunity for the clinical utility of Notch ligands in the expansion of primitive cells capable of hematopoietic reconstitution.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11067884-10075489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11067884-10079256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11067884-10190905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11067884-10194420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11067884-10221902,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11067884-10372105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11067884-10656756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11067884-10669757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11067884-10688781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11067884-10688783,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11067884-10688785,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11067884-10779426,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11067884-10784449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11067884-1372131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11067884-7512837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11067884-7541111,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11067884-7697721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11067884-8676076,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11067884-8761303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11067884-8917536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11067884-9144235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11067884-9245493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11067884-9254660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11067884-9275212,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11067884-9462510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11067884-9596653,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11067884-9716576,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11067884-9920832
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Notch,
http://linkedlifedata.com/resource/pubmed/chemical/Serrate proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
6
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pubmed:volume |
192
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1365-72
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11067884-Animals,
pubmed-meshheading:11067884-Antigens, CD,
pubmed-meshheading:11067884-Blood Cell Count,
pubmed-meshheading:11067884-Calcium-Binding Proteins,
pubmed-meshheading:11067884-Cell Division,
pubmed-meshheading:11067884-Cell Survival,
pubmed-meshheading:11067884-Cells, Cultured,
pubmed-meshheading:11067884-Cytokines,
pubmed-meshheading:11067884-Flow Cytometry,
pubmed-meshheading:11067884-Gene Expression,
pubmed-meshheading:11067884-Growth Substances,
pubmed-meshheading:11067884-Hematopoiesis,
pubmed-meshheading:11067884-Hematopoietic Stem Cell Transplantation,
pubmed-meshheading:11067884-Hematopoietic Stem Cells,
pubmed-meshheading:11067884-Humans,
pubmed-meshheading:11067884-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:11067884-Ligands,
pubmed-meshheading:11067884-Membrane Proteins,
pubmed-meshheading:11067884-Mice,
pubmed-meshheading:11067884-Mice, Inbred NOD,
pubmed-meshheading:11067884-Mice, SCID,
pubmed-meshheading:11067884-Protein Binding,
pubmed-meshheading:11067884-Proteins,
pubmed-meshheading:11067884-RNA, Messenger,
pubmed-meshheading:11067884-Receptors, Notch
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pubmed:year |
2000
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pubmed:articleTitle |
The notch ligand jagged-1 represents a novel growth factor of human hematopoietic stem cells.
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pubmed:affiliation |
John P. Robarts Research Institute, Developmental Stem Cell Biology, and the Department of Microbiology and Immunology, The University of Western Ontario, London, Ontario, Canada.
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