Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2000-12-1
pubmed:abstractText
Quantitation of cytokine production is a valuable adjunct to standard immunologic assays in defining several pathologic processes. Nevertheless, there is little agreement about which tissues should be assayed, which type of assay should be performed, and which stimulation protocol should be used. As these types of assays enter the clinical arena, there is need for standardization. There is also a need to maximize the amount of information which may be derived from a single sample. We compared secreted interleukin 4 (IL-4), IL-2, IL-6, tumor necrosis factor alpha (TNF-alpha), and gamma interferon proteins as measured by enzyme-linked immunosorbent assay with intracellular cytokine production (IL-2 and gamma interferon) as detected by flow cytometry and quantitative competitive PCR for IL-2, IL-4, TNF-alpha, and gamma interferon mRNA and cDNA. Results from unstimulated cells and cells stimulated with phorbol myristate acetate, phytohemagglutinin, and phorbol myristate acetate plus phytohemagglutin were compared. All three methodologies detected significant stimulation of cytokine production. The combination of phytohemagglutinin and phorbol myristate acetate was overall the most-potent stimulus.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11063499-10092835, http://linkedlifedata.com/resource/pubmed/commentcorrection/11063499-10450718, http://linkedlifedata.com/resource/pubmed/commentcorrection/11063499-1384862, http://linkedlifedata.com/resource/pubmed/commentcorrection/11063499-1519173, http://linkedlifedata.com/resource/pubmed/commentcorrection/11063499-1655771, http://linkedlifedata.com/resource/pubmed/commentcorrection/11063499-1690213, http://linkedlifedata.com/resource/pubmed/commentcorrection/11063499-2481313, http://linkedlifedata.com/resource/pubmed/commentcorrection/11063499-7561117, http://linkedlifedata.com/resource/pubmed/commentcorrection/11063499-8565269, http://linkedlifedata.com/resource/pubmed/commentcorrection/11063499-8763814, http://linkedlifedata.com/resource/pubmed/commentcorrection/11063499-8800688, http://linkedlifedata.com/resource/pubmed/commentcorrection/11063499-8856362, http://linkedlifedata.com/resource/pubmed/commentcorrection/11063499-8982362, http://linkedlifedata.com/resource/pubmed/commentcorrection/11063499-8985195, http://linkedlifedata.com/resource/pubmed/commentcorrection/11063499-9111542, http://linkedlifedata.com/resource/pubmed/commentcorrection/11063499-9462922, http://linkedlifedata.com/resource/pubmed/commentcorrection/11063499-9550432, http://linkedlifedata.com/resource/pubmed/commentcorrection/11063499-9605988, http://linkedlifedata.com/resource/pubmed/commentcorrection/11063499-9617844, http://linkedlifedata.com/resource/pubmed/commentcorrection/11063499-9717970, http://linkedlifedata.com/resource/pubmed/commentcorrection/11063499-9769110, http://linkedlifedata.com/resource/pubmed/commentcorrection/11063499-9825846, http://linkedlifedata.com/resource/pubmed/commentcorrection/11063499-9831384, http://linkedlifedata.com/resource/pubmed/commentcorrection/11063499-9865320, http://linkedlifedata.com/resource/pubmed/commentcorrection/11063499-9874670, http://linkedlifedata.com/resource/pubmed/commentcorrection/11063499-9895401
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1071-412X
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
920-4
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:11063499-Adult, pubmed-meshheading:11063499-Cytokines, pubmed-meshheading:11063499-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:11063499-Flow Cytometry, pubmed-meshheading:11063499-Gene Expression, pubmed-meshheading:11063499-Humans, pubmed-meshheading:11063499-Interferon-gamma, pubmed-meshheading:11063499-Interleukin-2, pubmed-meshheading:11063499-Interleukin-4, pubmed-meshheading:11063499-Leukocytes, Mononuclear, pubmed-meshheading:11063499-Lymphocyte Activation, pubmed-meshheading:11063499-Middle Aged, pubmed-meshheading:11063499-Phytohemagglutinins, pubmed-meshheading:11063499-Polymerase Chain Reaction, pubmed-meshheading:11063499-RNA, Messenger, pubmed-meshheading:11063499-Tetradecanoylphorbol Acetate, pubmed-meshheading:11063499-Tumor Necrosis Factor-alpha
pubmed:year
2000
pubmed:articleTitle
Measurement of cytokine secretion, intracellular protein expression, and mRNA in resting and stimulated peripheral blood mononuclear cells.
pubmed:affiliation
Division of Immunologic and Infectious Diseases, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania 19104, USA. sullivak@mail.med.upenn.edu
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't