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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
2001-5-25
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pubmed:databankReference |
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pubmed:abstractText |
Two cDNAs encoding novel K(+) channels, THIK-1 and THIK-2 (tandem pore domain halothane inhibited K(+) channel), were isolated from rat brain. The proteins of 405 and 430 amino acids were 58% identical to each other. Homology analysis showed that the novel channels form a separate subfamily among tandem pore domain K(+) channels. The genes of the human orthologs were identified as human genomic data base entries. They possess one intron each and were assigned to chromosomal region 14q24.1-14q24.3 (human (h) THIK-1) and 2p22-2p21 (hTHIK-2). In rat (r), THIK-1 (rTHIK-1) is expressed ubiquitously; rTHIK-2 expression was found in several tissues including brain and kidney. In situ hybridization of brain slices showed that rTHIK-2 is strongly expressed in most brain regions, whereas rTHIK-1 expression is more restricted. Heterologous expression of rTHIK-1 in Xenopus oocytes revealed a K(+) channel displaying weak inward rectification in symmetrical K(+) solution. The current was enhanced by arachidonic acid and inhibited by halothane. rTHIK-2 did not functionally express. Confocal microscopy of oocytes injected with green fluorescent protein-tagged rTHIK-1 or rTHIK-2 showed that both channel subunits are targeted to the outer membrane. However, coinjection of rTHIK-2 did not affect the currents induced by rTHIK-1, indicating that the two channel subunits do not form heteromers.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anesthetics, Inhalation,
http://linkedlifedata.com/resource/pubmed/chemical/Anions,
http://linkedlifedata.com/resource/pubmed/chemical/Arachidonic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Halothane,
http://linkedlifedata.com/resource/pubmed/chemical/Luminescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels, Tandem Pore...,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
9
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pubmed:volume |
276
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7302-11
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11060316-Amino Acid Sequence,
pubmed-meshheading:11060316-Anesthetics, Inhalation,
pubmed-meshheading:11060316-Animals,
pubmed-meshheading:11060316-Anions,
pubmed-meshheading:11060316-Arachidonic Acid,
pubmed-meshheading:11060316-Base Sequence,
pubmed-meshheading:11060316-Brain,
pubmed-meshheading:11060316-Chromosome Mapping,
pubmed-meshheading:11060316-Chromosomes, Human, Pair 14,
pubmed-meshheading:11060316-Chromosomes, Human, Pair 2,
pubmed-meshheading:11060316-Cloning, Molecular,
pubmed-meshheading:11060316-DNA, Complementary,
pubmed-meshheading:11060316-Electrophysiology,
pubmed-meshheading:11060316-Green Fluorescent Proteins,
pubmed-meshheading:11060316-Halothane,
pubmed-meshheading:11060316-Humans,
pubmed-meshheading:11060316-Hydrogen-Ion Concentration,
pubmed-meshheading:11060316-In Situ Hybridization,
pubmed-meshheading:11060316-Introns,
pubmed-meshheading:11060316-Kidney,
pubmed-meshheading:11060316-Liver,
pubmed-meshheading:11060316-Luminescent Proteins,
pubmed-meshheading:11060316-Microscopy, Confocal,
pubmed-meshheading:11060316-Molecular Sequence Data,
pubmed-meshheading:11060316-Phylogeny,
pubmed-meshheading:11060316-Potassium,
pubmed-meshheading:11060316-Potassium Channels,
pubmed-meshheading:11060316-Potassium Channels, Tandem Pore Domain,
pubmed-meshheading:11060316-Rats,
pubmed-meshheading:11060316-Recombinant Proteins,
pubmed-meshheading:11060316-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11060316-Sequence Homology, Amino Acid,
pubmed-meshheading:11060316-Stomach,
pubmed-meshheading:11060316-Tissue Distribution,
pubmed-meshheading:11060316-Xenopus
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pubmed:year |
2001
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pubmed:articleTitle |
THIK-1 and THIK-2, a novel subfamily of tandem pore domain K+ channels.
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pubmed:affiliation |
Institut für Normale und Pathologische Physiologie, Göttingen, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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