Linked Life Data

11060053

Source:http://linkedlifedata.com/resource/pubmed/id/11060053

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2000-11-17
pubmed:abstractText
We examined the efficiency of detection of immunoglobulin M (IgM) antibodies to a 35-kDa antigen (P35) of Toxoplasma gondii for serodiagnosis of acute infection in pregnant women. A double-sandwich enzyme-linked immunosorbent assay (ELISA) with recombinant P35 antigen (P35-IgM-ELISA) was used for this purpose. On the basis of the clinical history and the combination of results from the toxoplasma serological profile (Sabin-Feldman dye test, conventional IgM and IgA ELISAs, and the differential agglutination test), the patients were classified into three groups: group I, status suggestive of recently acquired infection; group II, status suggestive of infection acquired in the distant past; group III, status suggestive of persisting IgM antibodies. Eighteen (90.0%) of 20 serum samples from group I patients were positive by the P35-IgM-ELISA, whereas none of the 33 serum samples from group II patients were positive. Only 4 (25.0%) of 16 serum samples from group III patients were positive by the P35-IgM-ELISA, whereas all these serum samples were positive by the conventional IgM ELISA. These results indicate that demonstration of IgM antibodies against P35 by the P35-IgM-ELISA is more specific for the acute stage of the infection than demonstration of IgM antibodies by the ELISA that uses a whole-lysate antigen preparation. Studies with sera obtained from four pregnant women who seroconverted (IgG and IgM antibodies) during pregnancy revealed that two of them became negative by the P35-IgM-ELISA between 4 and 6 months after seroconversion, whereas the conventional IgM ELISA titers remained highly positive. The P35-IgM-ELISA appears to be useful for differentiating recently acquired infection from those acquired in the distant past in pregnant women.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11060053-10618084, http://linkedlifedata.com/resource/pubmed/commentcorrection/11060053-10699010, http://linkedlifedata.com/resource/pubmed/commentcorrection/11060053-10973455, http://linkedlifedata.com/resource/pubmed/commentcorrection/11060053-1460660, http://linkedlifedata.com/resource/pubmed/commentcorrection/11060053-2192008, http://linkedlifedata.com/resource/pubmed/commentcorrection/11060053-2229374, http://linkedlifedata.com/resource/pubmed/commentcorrection/11060053-3528317, http://linkedlifedata.com/resource/pubmed/commentcorrection/11060053-6348083, http://linkedlifedata.com/resource/pubmed/commentcorrection/11060053-7007520, http://linkedlifedata.com/resource/pubmed/commentcorrection/11060053-7077100, http://linkedlifedata.com/resource/pubmed/commentcorrection/11060053-8086543, http://linkedlifedata.com/resource/pubmed/commentcorrection/11060053-8556476, http://linkedlifedata.com/resource/pubmed/commentcorrection/11060053-8705683, http://linkedlifedata.com/resource/pubmed/commentcorrection/11060053-8968902, http://linkedlifedata.com/resource/pubmed/commentcorrection/11060053-9399504, http://linkedlifedata.com/resource/pubmed/commentcorrection/11060053-9729528, http://linkedlifedata.com/resource/pubmed/commentcorrection/11060053-9823527
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3967-70
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Detection of immunoglobulin M antibodies to P35 antigen of Toxoplasma gondii for serodiagnosis of recently acquired infection in pregnant women.
pubmed:affiliation
Department of Immunology and Infectious Diseases, Research Institute, Palo Alto Medical Foundation, Palo Alto, California 94301, USA. ysuzuki@leland.stanford.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Evaluation Studies