Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2001-5-23
pubmed:databankReference
pubmed:abstractText
We have characterized NORPEG, a novel gene from human retinal pigment epithelial cells (ARPE-19), in which its expression is induced by all-trans-retinoic acid. Two transcripts ( approximately 3 and approximately 5 kilobases in size) have been detected for this gene, which is localized to chromosome band 5p13.2-13.3. Placenta and testis showed the highest level of expression among various human tissues tested. Six ankyrin repeats and a long coiled-coil domain are present in the predicted sequence of the NORPEG protein, which contains 980 amino acid residues. This approximately 110-kDa protein was transiently expressed in COS-7 cells as a FLAG fusion protein and immunolocalized to the cytoplasm. Confocal microscopic analysis of the NORPEG protein in ARPE-19 cells showed threadlike projections in the cytoplasm reminiscent of the cytoskeleton. Consistent with this localization, the expressed NORPEG protein showed resistance to solubilization by Triton X-100 and KCl. An ortholog of NORPEG characterized from mouse encoded a protein that showed 91% sequence similarity to the human NORPEG protein. The expression of Norpeg mRNA was detected in mouse embryo at embryonic day 9.5 by in situ hybridization, and the expression appears to be developmentally regulated. In adult mouse, the highest level of expression was detected in the seminiferous tubules of testis.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
26
pubmed:volume
276
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2831-40
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:11042181-Amino Acid Sequence, pubmed-meshheading:11042181-Animals, pubmed-meshheading:11042181-Cells, Cultured, pubmed-meshheading:11042181-Chromosome Mapping, pubmed-meshheading:11042181-Chromosomes, Human, Pair 5, pubmed-meshheading:11042181-Cytoskeletal Proteins, pubmed-meshheading:11042181-Eye Proteins, pubmed-meshheading:11042181-Fetal Proteins, pubmed-meshheading:11042181-Gene Expression Regulation, pubmed-meshheading:11042181-Gene Library, pubmed-meshheading:11042181-Humans, pubmed-meshheading:11042181-In Situ Hybridization, Fluorescence, pubmed-meshheading:11042181-Male, pubmed-meshheading:11042181-Mice, pubmed-meshheading:11042181-Molecular Sequence Data, pubmed-meshheading:11042181-Pigment Epithelium of Eye, pubmed-meshheading:11042181-Seminiferous Tubules, pubmed-meshheading:11042181-Sequence Homology, Amino Acid, pubmed-meshheading:11042181-Species Specificity, pubmed-meshheading:11042181-Tissue Distribution, pubmed-meshheading:11042181-Transcription Factors, pubmed-meshheading:11042181-Tretinoin
pubmed:year
2001
pubmed:articleTitle
Molecular characterization and developmental expression of NORPEG, a novel gene induced by retinoic acid.
pubmed:affiliation
Biochemistry Section, Laboratory of Retinal Cell and Molecular Biology, and the Immunology and Virology Section, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892-2740, USA. krishnan@helix.nih.gov
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't