Source:http://linkedlifedata.com/resource/pubmed/id/11032781
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2001-1-26
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| pubmed:abstractText |
Recent work from this laboratory has demonstrated that purinergic-mediated depolarization of human microglia inhibited a store-operated pathway for entry of Ca2+. We have used Fura-2 spectrofluorometry to investigate the effects on store-operated Ca2+ influx induced by replacement of NaCl with Na-gluconate in extracellular solutions. Three separate procedures were used to activate store-operated channels. Platelet activating factor (PAF) was used to generate a sustained influx of Ca2+ in standard physiological saline solution (PSS). The magnitude of this response was depressed by 70% after replacement of PSS with low Cl- PSS. A second procedure used ATP, initially applied in Ca2+-free PSS solution to deplete intracellular stores. The subsequent perfusion of PSS solution containing Ca2+ resulted in a large and sustained entry of Ca2+, which was inhibited by 75% with low Cl- PSS. The SERCA inhibitor cyclopiazonic acid (CPA) was used to directly deplete stores in zero-Ca2+ PSS. Following the introduction of PSS containing Ca2+, a maintained stores-operated influx of Ca2+ was evident which was inhibited by 77% in the presence of the low Cl- PSS. Ca2+ influx was linearly reduced with cell depolarization in elevated K+ (7.5 to 35 mM) suggesting that changes in external Cl- were manifest as altered electrical driving force for Ca2+ entry. However, 50 mM external KCl effectively eliminated divalent entry which may indicate inactivation of this pathway with high magnitudes of depolarization. Patch clamp studies showed low Cl-PSS to cause depolarizing shifts in both holding currents and reversal potentials of currents activated with voltage ramps. The results demonstrate that Cl- channels play an important role in regulating store-operated entry of Ca2+ in human microglia.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Anions,
http://linkedlifedata.com/resource/pubmed/chemical/Buffers,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Transporting ATPases,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Gluconates,
http://linkedlifedata.com/resource/pubmed/chemical/Indoles,
http://linkedlifedata.com/resource/pubmed/chemical/Ion Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet Activating Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium,
http://linkedlifedata.com/resource/pubmed/chemical/Sarcoplasmic Reticulum...,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium Chloride,
http://linkedlifedata.com/resource/pubmed/chemical/cyclopiazonic acid,
http://linkedlifedata.com/resource/pubmed/chemical/gluconic acid
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| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
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| pubmed:issn |
0143-4160
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
28
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
261-8
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| pubmed:dateRevised |
2010-11-18
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| pubmed:meshHeading |
pubmed-meshheading:11032781-Adenosine Triphosphate,
pubmed-meshheading:11032781-Anions,
pubmed-meshheading:11032781-Buffers,
pubmed-meshheading:11032781-Calcium,
pubmed-meshheading:11032781-Calcium Signaling,
pubmed-meshheading:11032781-Calcium-Transporting ATPases,
pubmed-meshheading:11032781-Cells, Cultured,
pubmed-meshheading:11032781-Embryo, Mammalian,
pubmed-meshheading:11032781-Enzyme Inhibitors,
pubmed-meshheading:11032781-Extracellular Space,
pubmed-meshheading:11032781-Gluconates,
pubmed-meshheading:11032781-Humans,
pubmed-meshheading:11032781-Indoles,
pubmed-meshheading:11032781-Ion Channels,
pubmed-meshheading:11032781-Microglia,
pubmed-meshheading:11032781-Patch-Clamp Techniques,
pubmed-meshheading:11032781-Platelet Activating Factor,
pubmed-meshheading:11032781-Potassium,
pubmed-meshheading:11032781-Sarcoplasmic Reticulum Calcium-Transporting ATPases,
pubmed-meshheading:11032781-Sodium Chloride
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| pubmed:year |
2000
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| pubmed:articleTitle |
Anion channels modulate store-operated calcium influx in human microglia.
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| pubmed:affiliation |
Department of Pharmacology and Therapeutics, Faculty of Medicine, The University of British Columbia, Vancouver, British Columbia, Canada. Mclarnon@interchange.ubc.ca
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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