Linked Life Data

11031690

Source:http://linkedlifedata.com/resource/pubmed/id/11031690

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2000-12-28
pubmed:abstractText
The rabies virus glycoprotein (G) gene of the highly neuroinvasive and neurotropic strains SHBRV-18, CVS-N2c, and CVS-B2c was introduced into the non-neuroinvasive and less neurotropic SN-10 strain to provide further insight into the role of G in the pathogenesis of rabies. Phenotypic analyses of the recombinant viruses revealed, as expected, that the neurotropism of a particular rabies virus strain was a function of its G. Nevertheless, the pathogenicity of the recombinant viruses was, in every case, markedly lower than that of the wild-type viruses suggesting that while the G dictates neurotropism, other viral attributes are also important in pathogenesis. The low pathogenicity of the recombinant viruses is at least in part due to a strong increase in transcription activity. On the other hand, the production of infectious virus by the R-SHB18 recombinant virus-infected cells was significantly delayed by comparison with SHBRV-18 wild-type virus infected-cells. Replacement of the R-SHB18 G cytoplasmic domain, transmembrane domain, and stem region with its SN-10 G counterparts neither results in a significant increase in budding efficiency nor an increase in pathogenicity. These results suggest that an optimal match of the cytoplasmic domain of G with the matrix protein may not be sufficient for maximal virus budding efficiency, which is evidently a major factor of virus pathogenicity. Our studies indicate that to maintain pathogenicity, the interactions between various structural elements of rabies virus must be highly conserved and the expression of viral proteins, in particular the G protein, must be strictly controlled.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1355-0284
pubmed:author
pubmed:issnType
Print
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
373-81
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:11031690-Amino Acid Sequence, pubmed-meshheading:11031690-Animals, pubmed-meshheading:11031690-Antigens, Viral, pubmed-meshheading:11031690-Cricetinae, pubmed-meshheading:11031690-Gene Expression Regulation, Viral, pubmed-meshheading:11031690-Genetic Techniques, pubmed-meshheading:11031690-Glycoproteins, pubmed-meshheading:11031690-Kidney, pubmed-meshheading:11031690-Membrane Proteins, pubmed-meshheading:11031690-Molecular Sequence Data, pubmed-meshheading:11031690-Neuroblastoma, pubmed-meshheading:11031690-Neurons, pubmed-meshheading:11031690-Phenotype, pubmed-meshheading:11031690-Plasmids, pubmed-meshheading:11031690-Protein Structure, Tertiary, pubmed-meshheading:11031690-RNA, Messenger, pubmed-meshheading:11031690-RNA, Viral, pubmed-meshheading:11031690-Rabies, pubmed-meshheading:11031690-Rabies Vaccines, pubmed-meshheading:11031690-Rabies virus, pubmed-meshheading:11031690-Transcription, Genetic, pubmed-meshheading:11031690-Tumor Cells, Cultured, pubmed-meshheading:11031690-Viral Envelope Proteins, pubmed-meshheading:11031690-Virulence
pubmed:year
2000
pubmed:articleTitle
Reinvestigation of the role of the rabies virus glycoprotein in viral pathogenesis using a reverse genetics approach.
pubmed:affiliation
Department of Microbiology and Immunology, Thomas Jefferson University, Philadelphia, PA 19107, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.