10970351

Source:http://linkedlifedata.com/resource/pubmed/id/10970351

Download in:

Switch to

Custom View

Named Graph Language Inference

Statements in which the resource exists as a subject.
Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0020792, umls-concept:C0085469, umls-concept:C0348026, umls-concept:C1022294, umls-concept:C1038817, umls-concept:C1038818, umls-concept:C1043514, umls-concept:C1292724
pubmed:issue	9
pubmed:dateCreated	2000-11-24
pubmed:databankReference	http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF143774, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF143775, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF143776, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF143777, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF143778, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF143779, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF143780, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF143781, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF143782, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF143783, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF143784, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF143785, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF143786, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF143787, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF143788, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF143789, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF143790, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF143791, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF143792, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF143793
pubmed:abstractText	Bacteria of the Burkholderia cepacia complex consist of five discrete genomic species, including genomovars I and III and three new species: Burkholderia multivorans (formerly genomovar II), Burkholderia stabilis (formerly genomovar IV), and Burkholderia vietnamiensis (formerly genomovar V). Strains of all five genomovars are capable of causing opportunistic human infection, and microbiological identification of these closely related species is difficult. The 16S rRNA gene (16S rDNA) and recA gene of these bacteria were examined in order to develop rapid tests for genomovar identification. Restriction fragment length polymorphism (RFLP) analysis of PCR-amplified 16S rDNA revealed sequence polymorphisms capable of identifying B. multivorans and B. vietnamiensis but insufficient to discriminate strains of B. cepacia genomovars I and III and B. stabilis. RFLP analysis of PCR-amplified recA demonstrated sufficient nucleotide sequence variation to enable separation of strains of all five B. cepacia complex genomovars. Complete recA nucleotide sequences were obtained for 20 strains representative of the diversity of the B. cepacia complex. Construction of a recA phylogenetic tree identified six distinct clusters (recA groups): B. multivorans, B. vietnamiensis, B. stabilis, genomovar I, and the subdivision of genomovar III isolates into two recA groups, III-A and III-B. Alignment of recA sequences enabled the design of PCR primers for the specific detection of each of the six latter recA groups. The recA gene was found on the largest chromosome within the genome of B. cepacia complex strains and, in contrast to the findings of a previous study, only a single copy of the gene was present. In conclusion, analysis of the recA gene of the B. cepacia complex provides a rapid and robust nucleotide sequence-based approach to identify and classify this taxonomically complex group of opportunistic pathogens.
pubmed:commentsCorrections	http://linkedlifedata.com/resource/pubmed/commentcorrection/10970351-10364586, http://linkedlifedata.com/resource/pubmed/commentcorrection/10970351-10488171, http://linkedlifedata.com/resource/pubmed/commentcorrection/10970351-10655415, http://linkedlifedata.com/resource/pubmed/commentcorrection/10970351-10698993, http://linkedlifedata.com/resource/pubmed/commentcorrection/10970351-2227456, http://linkedlifedata.com/resource/pubmed/commentcorrection/10970351-7476209, http://linkedlifedata.com/resource/pubmed/commentcorrection/10970351-7517389, http://linkedlifedata.com/resource/pubmed/commentcorrection/10970351-7527826, http://linkedlifedata.com/resource/pubmed/commentcorrection/10970351-7532166, http://linkedlifedata.com/resource/pubmed/commentcorrection/10970351-7537977, http://linkedlifedata.com/resource/pubmed/commentcorrection/10970351-7592482, http://linkedlifedata.com/resource/pubmed/commentcorrection/10970351-7984417, http://linkedlifedata.com/resource/pubmed/commentcorrection/10970351-8587109, http://linkedlifedata.com/resource/pubmed/commentcorrection/10970351-8801440, http://linkedlifedata.com/resource/pubmed/commentcorrection/10970351-8840786, http://linkedlifedata.com/resource/pubmed/commentcorrection/10970351-8858887, http://linkedlifedata.com/resource/pubmed/commentcorrection/10970351-8900054, http://linkedlifedata.com/resource/pubmed/commentcorrection/10970351-8940422, http://linkedlifedata.com/resource/pubmed/commentcorrection/10970351-9157133, http://linkedlifedata.com/resource/pubmed/commentcorrection/10970351-9254694, http://linkedlifedata.com/resource/pubmed/commentcorrection/10970351-9304858, http://linkedlifedata.com/resource/pubmed/commentcorrection/10970351-9336927, http://linkedlifedata.com/resource/pubmed/commentcorrection/10970351-9487711, http://linkedlifedata.com/resource/pubmed/commentcorrection/10970351-9705429, http://linkedlifedata.com/resource/pubmed/commentcorrection/10970351-9759550, http://linkedlifedata.com/resource/pubmed/commentcorrection/10970351-9782485
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/7505564
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Ribosomal, 16S, http://linkedlifedata.com/resource/pubmed/chemical/Rec A Recombinases
pubmed:status	MEDLINE
pubmed:month	Sep
pubmed:issn	0095-1137
pubmed:author	pubmed-author:Av-GayYY, pubmed-author:BischofJJ, pubmed-author:ByrneS KSK, pubmed-author:DaviesJ EJE, pubmed-author:MahenthiralingamEE, pubmed-author:RadomskaKK, pubmed-author:VandammePP
pubmed:issnType	Print
pubmed:volume	38
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	3165-73
pubmed:dateRevised	2009-11-18
pubmed:meshHeading	pubmed-meshheading:10970351-Burkholderia Infections, pubmed-meshheading:10970351-Burkholderia cepacia, pubmed-meshheading:10970351-Chromosome Mapping, pubmed-meshheading:10970351-DNA, Bacterial, pubmed-meshheading:10970351-Genes, rRNA, pubmed-meshheading:10970351-Humans, pubmed-meshheading:10970351-Molecular Sequence Data, pubmed-meshheading:10970351-Opportunistic Infections, pubmed-meshheading:10970351-Phylogeny, pubmed-meshheading:10970351-Polymerase Chain Reaction, pubmed-meshheading:10970351-Polymorphism, Restriction Fragment Length, pubmed-meshheading:10970351-RNA, Ribosomal, 16S, pubmed-meshheading:10970351-Rec A Recombinases, pubmed-meshheading:10970351-Sequence Analysis, DNA
pubmed:year	2000
pubmed:articleTitle	DNA-Based diagnostic approaches for identification of Burkholderia cepacia complex, Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis, and Burkholderia cepacia genomovars I and III.
pubmed:affiliation	Department of Pediatrics, University of British Columbia and British Columbia's Children's Hospital, British Columbia's Research Institute for Children's and Women's Health, Vancouver, British Columbia, Canada. MahenthiralingamE@cardiff.ac.uk
pubmed:publicationType	Journal Article, Research Support, Non-U.S. Gov't, Evaluation Studies