Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
18
pubmed:dateCreated
2000-9-26
pubmed:abstractText
We investigated the roles of fliF, fliS, flhB, fliQ, fliG, and fliI of Helicobacter pylori, predicted by homology to encode structural components of the flagellar basal body and export apparatus. Mutation of these genes resulted in nonmotile, nonflagellate strains. Western blot analysis showed that all the mutants had considerably reduced levels of both flagellin subunits and of FlgE, the flagellar hook protein. RNA slot blot hybridization showed reduced levels of flaA mRNA, indicating that transcription of the major flagellin gene is inhibited in the absence of the early components of the flagellar-assembly pathway. This is the first demonstration of a checkpoint in H. pylori flagellar assembly.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10960117-10049367, http://linkedlifedata.com/resource/pubmed/commentcorrection/10960117-10225855, http://linkedlifedata.com/resource/pubmed/commentcorrection/10960117-10234819, http://linkedlifedata.com/resource/pubmed/commentcorrection/10960117-1320607, http://linkedlifedata.com/resource/pubmed/commentcorrection/10960117-1435261, http://linkedlifedata.com/resource/pubmed/commentcorrection/10960117-1704004, http://linkedlifedata.com/resource/pubmed/commentcorrection/10960117-3937729, http://linkedlifedata.com/resource/pubmed/commentcorrection/10960117-3950447, http://linkedlifedata.com/resource/pubmed/commentcorrection/10960117-7627454, http://linkedlifedata.com/resource/pubmed/commentcorrection/10960117-7768796, http://linkedlifedata.com/resource/pubmed/commentcorrection/10960117-7891557, http://linkedlifedata.com/resource/pubmed/commentcorrection/10960117-8074885, http://linkedlifedata.com/resource/pubmed/commentcorrection/10960117-8235660, http://linkedlifedata.com/resource/pubmed/commentcorrection/10960117-8501031, http://linkedlifedata.com/resource/pubmed/commentcorrection/10960117-8698465, http://linkedlifedata.com/resource/pubmed/commentcorrection/10960117-8891207, http://linkedlifedata.com/resource/pubmed/commentcorrection/10960117-9023175, http://linkedlifedata.com/resource/pubmed/commentcorrection/10960117-9098027, http://linkedlifedata.com/resource/pubmed/commentcorrection/10960117-9231413, http://linkedlifedata.com/resource/pubmed/commentcorrection/10960117-9252185, http://linkedlifedata.com/resource/pubmed/commentcorrection/10960117-9259572, http://linkedlifedata.com/resource/pubmed/commentcorrection/10960117-9529052, http://linkedlifedata.com/resource/pubmed/commentcorrection/10960117-9882675, http://linkedlifedata.com/resource/pubmed/commentcorrection/10960117-9923682
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Flagellin, http://linkedlifedata.com/resource/pubmed/chemical/FlhB protein, Bacteria, http://linkedlifedata.com/resource/pubmed/chemical/FliQ protein, Bacteria, http://linkedlifedata.com/resource/pubmed/chemical/FliQ protein, E coli, http://linkedlifedata.com/resource/pubmed/chemical/FliS protein, Bacteria, http://linkedlifedata.com/resource/pubmed/chemical/Flif protein, Bacteria, http://linkedlifedata.com/resource/pubmed/chemical/Flig protein, Bacteria, http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Proton-Translocating ATPases, http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins, http://linkedlifedata.com/resource/pubmed/chemical/fliI protein, bacteria
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
182
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5274-7
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Mutational analysis of genes encoding the early flagellar components of Helicobacter pylori: evidence for transcriptional regulation of flagellin A biosynthesis.
pubmed:affiliation
Pathogen Molecular Biology and Biochemistry Unit, Department of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, United Kingdom.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't