Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
17
pubmed:dateCreated
2000-9-14
pubmed:databankReference
pubmed:abstractText
The RNA genome of Borna disease virus (BDV) shows extraordinary stability in persistently infected cell cultures. We performed bottleneck experiments in which virus populations from single infected cells were allowed to spread through cultures of uninfected cells and in which RNase protection assays were used to identify virus variants with mutations in a 535-nucleotide fragment of the M-G open reading frames. In one of the cell cultures, the major virus species (designated 2/1) was a variant with two point mutations in the G open reading frame. When fresh cells were infected with a low dose of a virus stock prepared from 2/1-containing cells, only a minority of the resulting persistently infected cultures contained detectable levels of the variant, whereas the others all seemed to contain wild-type virus. The BDV variant 2/1 remained stable in the various persistently infected cell cultures, indicating that the cells were resistant to superinfection by wild-type virus. Indeed, cells persistently infected with prototype BDV He/80 were also found to resist superinfection with strain V and vice versa. Our screen for mutations in the viral M and G genes of different rat-derived BDV virus stocks revealed that only one of four stocks believed to contain He/80 harbored virus with the original sequence. Two stocks mainly contained a novel virus variant with about 3% sequence divergence, whereas the fourth stock contained a mixture of both viruses. When the mixture was inoculated into the brains of newborn mice, the novel variant was preferentially amplified. These results provide evidence that the BDV genome is mutating more frequently than estimated from its invariant appearance in persistently infected cell cultures and that resistance to superinfection might strongly select against novel variants.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10933695-10226619, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933695-10227422, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933695-10400715, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933695-10446654, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933695-10515835, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933695-10602401, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933695-10622306, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933695-10823873, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933695-10900032, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933695-1402812, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933695-1454837, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933695-167114, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933695-2535733, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933695-2983944, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933695-2990100, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933695-3051132, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933695-3920840, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933695-4287698, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933695-6772932, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933695-7041255, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933695-7789150, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933695-7789151, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933695-7856315, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933695-7906311, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933695-8254777, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933695-8416380, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933695-9343347, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933695-9421127, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933695-9535888, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933695-9557728, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933695-9746029, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933695-9807733, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933695-9811743
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
74
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7878-83
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Sequence variability of Borna disease virus: resistance to superinfection may contribute to high genome stability in persistently infected cells.
pubmed:affiliation
Department of Virology, Institute for Medical Microbiology and Hygiene, University of Freiburg, D-79104 Freiburg, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't