Source:http://linkedlifedata.com/resource/pubmed/id/10918193
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
2000-8-18
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| pubmed:abstractText |
An association between codon-72 p53 polymorphism and risk of human papillomavirus (HPV)-induced cervical cancer has been found recently, but it has been difficult to replicate. In this study, we assess the impact of inter-laboratory variation in p53 genotyping on the validity of the proposed association. DNA specimens were randomly selected from 54 invasive, squamous cell carcinoma cases, 52 HPV-negative, and 39 HPV-positive controls from a previous case-control study in Brazil. Codon-72 polymorphism was blindly analyzed in three different laboratories. We calculated age- and race-adjusted odds ratios (OR) and 95% confidence intervals (CI) using logistic regression for gauging the association between p53 polymorphism and cervical cancer risk. The proportions of the Arg/Arg, Arg/Pro, and Pro/Pro genotypes varied substantially among laboratories with Kappa coefficients in the 0.49-0.63 range. When disagreement between labs was allowed, the OR for the Arg/Arg genotype, compared to other forms, was as low as 1.5 (95% CI: 0.5-3. 9). In contrast, the OR increased to 8.0 (95% CI: 2.3-28.5) after exclusion of discordant genotypes. Restricting the comparison to HPV-positive controls increased the magnitude of the relation appreciably. After exclusion of all discordant diagnoses, the OR was 21.5 (95% CI: 3.4-137.8), whereas with disagreed genotypes the association was not significant (OR = 2.9, 95% CI: 0.7-11.9). Homozygous codon-72 p53-Arg apparently confers a higher susceptibility to HPV-associated cervical tumorigenesis. However, exposure misclassification consequent to inter-laboratory variation in protocols may affect the ability to detect the association.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
0020-7136
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2000 Wiley-Liss, Inc.
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| pubmed:issnType |
Print
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| pubmed:day |
15
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| pubmed:volume |
87
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
528-33
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| pubmed:dateRevised |
2007-7-24
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| pubmed:meshHeading |
pubmed-meshheading:10918193-Adult,
pubmed-meshheading:10918193-Aged,
pubmed-meshheading:10918193-Arginine,
pubmed-meshheading:10918193-Case-Control Studies,
pubmed-meshheading:10918193-Cocarcinogenesis,
pubmed-meshheading:10918193-Codon,
pubmed-meshheading:10918193-Female,
pubmed-meshheading:10918193-Genes, p53,
pubmed-meshheading:10918193-Genotype,
pubmed-meshheading:10918193-Humans,
pubmed-meshheading:10918193-Middle Aged,
pubmed-meshheading:10918193-Observer Variation,
pubmed-meshheading:10918193-Papillomaviridae,
pubmed-meshheading:10918193-Papillomavirus Infections,
pubmed-meshheading:10918193-Polymorphism, Genetic,
pubmed-meshheading:10918193-Reproducibility of Results,
pubmed-meshheading:10918193-Risk Factors,
pubmed-meshheading:10918193-Tumor Suppressor Protein p53,
pubmed-meshheading:10918193-Tumor Virus Infections,
pubmed-meshheading:10918193-Uterine Cervical Neoplasms
|
| pubmed:year |
2000
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| pubmed:articleTitle |
P53 polymorphism in codon 72 and risk of human papillomavirus-induced cervical cancer: effect of inter-laboratory variation.
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| pubmed:affiliation |
Department of Oncology, McGill University, Montreal, Canada.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|