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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
16
pubmed:dateCreated
2000-8-28
pubmed:abstractText
Sik (mouse Src-related intestinal kinase) and its orthologue BRK (human breast tumor kinase) are intracellular tyrosine kinases that are distantly related to the Src family and have a similar structure, but they lack the myristoylation signal. Here we demonstrate that Sik and BRK associate with the RNA binding protein Sam68 (Src associated during mitosis, 68 kDa). We found that Sik interacts with Sam68 through its SH3 and SH2 domains and that the proline-rich P3 region of Sam68 is required for Sik and BRK SH3 binding. In the transformed HT29 adenocarcinoma cell cell line, endogenous BRK and Sam68 colocalize in Sam68-SLM nuclear bodies (SNBs), while transfected Sik and Sam68 are localized diffusely in the nucleoplasm of nontransformed NMuMG mammary epithelial cells. Transfected Sik phosphorylates Sam68 in SNBs in HT29 cells and in the nucleoplasm of NMuMG cells. In functional studies, expression of Sik abolished the ability of Sam68 to bind RNA and act as a cellular Rev homologue. While Sam68 is a substrate for Src family kinases during mitosis, Sik/BRK is the first identified tyrosine kinase that can phosphorylate Sam68 and regulate its activity within the nucleus, where it resides during most of the cell cycle.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-10371501, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-10430081, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-10437794, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-10453476, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-10473643, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-1374686, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-1852137, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-2217212, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-2683363, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-3982419, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-7511210, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-7512694, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-7512695, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-7516469, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-7530715, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-7537265, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-7601353, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-7680959, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-7685656, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-7799925, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-7838533, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-7985231, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-8036022, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-8208550, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-8247543, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-8405383, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-8464704, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-8490650, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-8589716, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-8618911, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-8791414, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-8995379, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-9010224, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-9013542, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-9118803, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-9185712, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-9229110, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-9266966, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-9315091, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-9315629, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-9341174, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-9405638, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-9433137, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-9464824, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-9671495, http://linkedlifedata.com/resource/pubmed/commentcorrection/10913193-9804767
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0270-7306
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6114-26
pubmed:dateRevised
2010-12-7
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Sik (BRK) phosphorylates Sam68 in the nucleus and negatively regulates its RNA binding ability.
pubmed:affiliation
Departments of Molecular Genetics and Medicine, University of Illinois at Chicago, Chicago, Illinois 60607, USA.
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