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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2000-8-31
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pubmed:abstractText |
This study demonstrates for the first time the effects of IL-1beta on the regulation of protein production as well as MUC2 gene transcription in cultured human airway epithelial cells. The effect of IL-1beta on the regulation of MUC2 protein was determined by flow cytometric analysis. The expression level of MUC2 induced by IL-1beta increased in a dose-dependent manner. MUC2 transcripts were detected after 2 h of exposure to IL-1beta and reached maximal level after 8 h. Actinomycin D experiments indicated that the IL-1beta-mediated MUC2 expression was controlled by transcriptional regulation. Both RT-PCR and FACS analysis showed that budesonide concomitantly attenuated IL-1beta mediated MUC2 gene as well as protein production levels. Use of the glucocorticoid receptor antagonist, RU-486, restored the inhibitory effect of budesonide on the IL-1beta-mediated MUC2 protein as well as gene. The data suggest that IL-1beta up-regulates MUC2 gene by transcriptional regulation and that budesonide suppresses the IL-1beta-medicated MUC2 expression via decreased transcriptional activation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bronchodilator Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Budesonide,
http://linkedlifedata.com/resource/pubmed/chemical/Dactinomycin,
http://linkedlifedata.com/resource/pubmed/chemical/Hormone Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/MUC2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Mifepristone,
http://linkedlifedata.com/resource/pubmed/chemical/Mucin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Mucins,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleic Acid Synthesis Inhibitors
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2000 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
21
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pubmed:volume |
274
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
112-6
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10903904-Bronchodilator Agents,
pubmed-meshheading:10903904-Budesonide,
pubmed-meshheading:10903904-Cell Separation,
pubmed-meshheading:10903904-Dactinomycin,
pubmed-meshheading:10903904-Dose-Response Relationship, Drug,
pubmed-meshheading:10903904-Flow Cytometry,
pubmed-meshheading:10903904-Gene Expression Regulation,
pubmed-meshheading:10903904-Hormone Antagonists,
pubmed-meshheading:10903904-Humans,
pubmed-meshheading:10903904-Interleukin-1,
pubmed-meshheading:10903904-Lung,
pubmed-meshheading:10903904-Mifepristone,
pubmed-meshheading:10903904-Mucin-2,
pubmed-meshheading:10903904-Mucins,
pubmed-meshheading:10903904-Nucleic Acid Synthesis Inhibitors,
pubmed-meshheading:10903904-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:10903904-Time Factors,
pubmed-meshheading:10903904-Transcription, Genetic,
pubmed-meshheading:10903904-Transcriptional Activation,
pubmed-meshheading:10903904-Tumor Cells, Cultured,
pubmed-meshheading:10903904-Up-Regulation
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pubmed:year |
2000
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pubmed:articleTitle |
Regulation of IL-1beta-mediated MUC2 gene in NCI-H292 human airway epithelial cells.
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pubmed:affiliation |
Department of Otorhinolaryngology, Yeungnam University, Taegu, 705-717, South Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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