Linked Life Data

10903171

Source:http://linkedlifedata.com/resource/pubmed/id/10903171

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
16
pubmed:dateCreated
2000-9-19
pubmed:abstractText
Development of the gastrointestinal (GI) tract depends on reciprocal epithelial-mesenchymal cell signaling. Here, we demonstrate a role for platelet-derived growth factor-A (PDGF-A) and its receptor, PDGFR-(alpha), in this process. Mice lacking PDGF-A or PDGFR-(alpha) were found to develop an abnormal GI mucosal lining, including fewer and misshapen villi and loss of pericryptal mesenchyme. Onset of villus morphogenesis correlated with the formation of clusters of PDGFR-(alpha) positive cells, 'villus clusters', which remained located at the tip of the mesenchymal core of the growing villus. Lack of PDGF-A or PDGFR-(alpha) resulted in progressive depletion of PDGFR-(alpha) positive mesenchymal cells, the formation of fewer villus clusters, and premature expression of smooth muscle actin (SMA) in the villus mesenchyme. We found that the villus clusters were postmitotic, expressed BMP-2 and BMP-4, and that their formation correlated with downregulated DNA synthesis in adjacent intestinal epithelium. We propose a model in which villus morphogenesis is initiated as a result of aggregation of PDGFR-(&agr;) positive cells into cell clusters that subsequently function as mesenchymal centers of signaling to the epithelium. The role of PDGF-A seems to be to secure renewal of PDGFR-(alpha) positive cells when they are consumed in the initial rounds of cluster formation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0950-1991
pubmed:author
pubmed:issnType
Print
pubmed:volume
127
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3457-66
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:10903171-Animals, pubmed-meshheading:10903171-Bone Morphogenetic Protein 2, pubmed-meshheading:10903171-Bone Morphogenetic Protein 4, pubmed-meshheading:10903171-Bone Morphogenetic Proteins, pubmed-meshheading:10903171-Cell Differentiation, pubmed-meshheading:10903171-Cell Division, pubmed-meshheading:10903171-Digestive System, pubmed-meshheading:10903171-Gene Expression Profiling, pubmed-meshheading:10903171-Intestines, pubmed-meshheading:10903171-Mesoderm, pubmed-meshheading:10903171-Mice, pubmed-meshheading:10903171-Mice, Inbred C57BL, pubmed-meshheading:10903171-Mice, Knockout, pubmed-meshheading:10903171-Microvilli, pubmed-meshheading:10903171-Morphogenesis, pubmed-meshheading:10903171-Muscle, Smooth, pubmed-meshheading:10903171-Platelet-Derived Growth Factor, pubmed-meshheading:10903171-Receptor, Platelet-Derived Growth Factor alpha, pubmed-meshheading:10903171-Transforming Growth Factor beta
pubmed:year
2000
pubmed:articleTitle
Abnormal gastrointestinal development in PDGF-A and PDGFR-(alpha) deficient mice implicates a novel mesenchymal structure with putative instructive properties in villus morphogenesis.
pubmed:affiliation
Department of Medical Biochemistry, Göteborg University, Medicinaregatan 9A, Box 440, SE 405 30 Göteborg, Sweden. Linda. Karlsson@medkem.gu.se
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't