Linked Life Data

10899941

Source:http://linkedlifedata.com/resource/pubmed/id/10899941

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2000-8-17
pubmed:abstractText
The neural cell adhesion molecule NCAM is involved in axonal outgrowth and target recognition in the developing nervous system. In vitro, NCAM-NCAM binding has been shown to induce neurite outgrowth, presumably through an activation of fibroblast growth factor receptors (FGFRs). We have recently identified a neuritogenic ligand, termed the C3 peptide, of the first immunoglobulin (lg) module of NCAM using a combinatorial library of synthetic peptides. Here we investigate whether stimulation of neurite outgrowth by this synthetic ligand of NCAM involves FGFRs. In primary cultures of cerebellar neurons from wild-type mice, the C3 peptide stimulated neurite outgrowth. This response was virtually absent in cultures of cerebellar neurons from transgenic mice expressing a dominant-negative form of the FGFR1. Likewise, in PC12E2 cells transiently expressing a dominant-negative form of the mouse FGFR1, induction of neurites by the C3 peptide was abrogated. These findings suggest that the neuritogenic effect of the C3 peptide requires the presence of functional FGFRs and support the hypothesis that FGFRs are essential in cell adhesion molecule-stimulated neurite outgrowth. The C3 peptide appears to stimulate neurite outgrowth by specifically activating an NCAM-FGFR-dependent signaling cascade and may therefore be of considerable interest as a tool for the determination of NCAM-dependent neurite outgrowth as well as a potential drug capable of promoting outgrowth and regeneration of NCAM-responsive axons.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0022-3042
pubmed:author
pubmed:issnType
Print
pubmed:volume
75
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
665-71
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:10899941-3T3 Cells, pubmed-meshheading:10899941-Amino Acid Sequence, pubmed-meshheading:10899941-Animals, pubmed-meshheading:10899941-Cells, Cultured, pubmed-meshheading:10899941-Cerebellum, pubmed-meshheading:10899941-L Cells (Cell Line), pubmed-meshheading:10899941-Ligands, pubmed-meshheading:10899941-Mice, pubmed-meshheading:10899941-Mice, Transgenic, pubmed-meshheading:10899941-Neural Cell Adhesion Molecules, pubmed-meshheading:10899941-Neurites, pubmed-meshheading:10899941-Neurons, pubmed-meshheading:10899941-Oligopeptides, pubmed-meshheading:10899941-PC12 Cells, pubmed-meshheading:10899941-Rats, pubmed-meshheading:10899941-Receptor, Fibroblast Growth Factor, Type 1, pubmed-meshheading:10899941-Receptor Protein-Tyrosine Kinases, pubmed-meshheading:10899941-Receptors, Fibroblast Growth Factor, pubmed-meshheading:10899941-Recombinant Proteins, pubmed-meshheading:10899941-Transfection
pubmed:year
2000
pubmed:articleTitle
Neurite outgrowth induced by a synthetic peptide ligand of neural cell adhesion molecule requires fibroblast growth factor receptor activation.
pubmed:affiliation
The Protein Laboratory, Institute of Molecular Pathology, Panum Institute, Copenhagen, Denmark. lcr@plab.ku.dk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't