Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
15
pubmed:dateCreated
2000-8-10
pubmed:abstractText
Human cytomegalovirus (CMV) is the leading cause of prenatal viral infection. Affected infants may suffer intrauterine growth retardation and serious neurologic impairment. Analysis of spontaneously aborted conceptuses shows that CMV infects the placenta before the embryo or fetus. In the human hemochorial placenta, maternal blood directly contacts syncytiotrophoblasts that cover chorionic villi and cytotrophoblasts that invade uterine vessels, suggesting possible routes for CMV transmission. To test this hypothesis, we exposed first-trimester chorionic villi and isolated cytotrophoblasts to CMV in vitro. In chorionic villi, syncytiotrophoblasts did not become infected, although clusters of underlying cytotrophoblasts expressed viral proteins. In chorionic villi that were infected with CMV in utero, syncytiotrophoblasts were often spared, whereas cytotrophoblasts and other cells of the villous core expressed viral proteins. Isolated cytotrophoblasts were also permissive for CMV replication in vitro; significantly, infection subsequently impaired the cytotrophoblasts' ability to differentiate and invade. These results suggest two possible routes of CMV transmission to the fetus: (i) across syncytiotrophoblasts with subsequent infection of the underlying cytotrophoblasts and (ii) via invasive cytotrophoblasts within the uterine wall. Furthermore, the observation that CMV infection impairs critical aspects of cytotrophoblast function offers testable hypotheses for explaining the deleterious effects of this virus on pregnancy outcome.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-1005030, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-1310525, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-1312642, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-1330874, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-1370295, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-1470605, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-1649872, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-165244, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-1847399, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-1849141, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-2326636, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-2474556, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-2981937, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-2984642, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-2991565, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-2993430, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-2993442, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-3020264, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-3029241, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-3033505, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-3181993, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-3980589, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-4327939, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-4373898, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-6278309, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-6324716, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-6324849, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-7529679, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-7609050, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-7680671, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-7692667, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-7706718, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-7751685, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-7756493, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-7844361, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-7964511, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-7979967, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-7985020, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-7991550, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-7995333, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-8236822, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-8380609, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-8760807, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-8766556, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-8766579, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-8871627, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-9049319, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-9151786, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-9151787, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-9220166, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-9335340, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-9403732, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-9520471, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-9573266, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-9593002, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-9637505, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-9687527, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-9696860, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-9818178, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-9833956, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-9882507, http://linkedlifedata.com/resource/pubmed/commentcorrection/10888620-9915996
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
74
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6808-20
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
More...