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pubmed-article:10880825pubmed:abstractTextA simple system for cutting dendrites of single neurons without damaging the viability of the cell is described. The system utilizes a rapidly vibrating (100 Hz) micropipette (<1 microm tip diameter), which is dragged under direct visual control across the dendrite of a selected neuron. We used this system in a thin slice preparation to dissect the apical dendrites of rat hippocampal CA1 pyramidal cells. We then used the patch-clamp technique in the whole-cell configuration to record from the isolated somata of these cells and to inject a dye into them. Both a functional and an anatomical disconnection of the dendrites from their somata were verified.lld:pubmed
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pubmed-article:10880825pubmed:authorpubmed-author:YaariYYlld:pubmed
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pubmed-article:10880825pubmed:pagination119-22lld:pubmed
pubmed-article:10880825pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:10880825pubmed:year2000lld:pubmed
pubmed-article:10880825pubmed:articleTitleA novel technique for micro-dissection of neuronal processes.lld:pubmed
pubmed-article:10880825pubmed:affiliationDepartment of Physiology, Hebrew University-Hadassah School of Medicine, PO Box 12272, 91120, Jerusalem, Israel.lld:pubmed
pubmed-article:10880825pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:10880825pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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