Linked Life Data

10880825

Source:http://linkedlifedata.com/resource/pubmed/id/10880825

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2000-8-24
pubmed:abstractText
A simple system for cutting dendrites of single neurons without damaging the viability of the cell is described. The system utilizes a rapidly vibrating (100 Hz) micropipette (<1 microm tip diameter), which is dragged under direct visual control across the dendrite of a selected neuron. We used this system in a thin slice preparation to dissect the apical dendrites of rat hippocampal CA1 pyramidal cells. We then used the patch-clamp technique in the whole-cell configuration to record from the isolated somata of these cells and to inject a dye into them. Both a functional and an anatomical disconnection of the dendrites from their somata were verified.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0165-0270
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
98
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
119-22
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
A novel technique for micro-dissection of neuronal processes.
pubmed:affiliation
Department of Physiology, Hebrew University-Hadassah School of Medicine, PO Box 12272, 91120, Jerusalem, Israel.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't