Linked Life Data

10861844

Source:http://linkedlifedata.com/resource/pubmed/id/10861844

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2000-8-24
pubmed:abstractText
Osteopontin (OPN) is a secreted glycophosphoprotein which induces migration of mammary carcinoma cells, and has been implicated in the malignancy of breast carcinoma. Hepatocyte growth factor (HGF) induces cell migration of several mammary epithelial cell (MEC) lines, via activation of its cognate receptor (Met). This study examines the mechanism of OPN-induced MEC migration, in terms of the cell surface integrins involved and induction of the HGF/Met pathway. Three different MEC cell lines were used, representing different stages of tumor progression: 21PT, non-tumorigenic; 21NT, tumorigenic; non-metastatic; and MDA-MB-435, tumorigenic, highly metastatic. Human recombinant OPN was found to induce the migration of all three lines. OPN-induced migration of 21PT and 21NT cells was alphavbeta5 and beta1-integrin dependent, and alphavbeta3-independent, while that of MDA-MB-435 cells was alphavbeta3-dependent. HGF also induced migration of all three cell lines, and a synergistic response was seen to HGF and OPN together. The increased migration response to OPN was found to be associated with an initial increase in Met kinase activity (within 30 min), followed by an increase in Met mRNA and protein expression. OPN-induced cell migration is thus mediated by different cell surface integrins in MEC lines representing different stages of progression, and involves activation of the HGF receptor, Met.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0730-2312
pubmed:author
pubmed:copyrightInfo
Copyright 2000 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:day
6
pubmed:volume
78
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
465-75
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:10861844-Blotting, Northern, pubmed-meshheading:10861844-Blotting, Western, pubmed-meshheading:10861844-Breast, pubmed-meshheading:10861844-Breast Neoplasms, pubmed-meshheading:10861844-Cell Movement, pubmed-meshheading:10861844-Cells, Cultured, pubmed-meshheading:10861844-Epithelial Cells, pubmed-meshheading:10861844-Female, pubmed-meshheading:10861844-Gene Expression, pubmed-meshheading:10861844-Humans, pubmed-meshheading:10861844-Integrins, pubmed-meshheading:10861844-Osteopontin, pubmed-meshheading:10861844-Phosphoproteins, pubmed-meshheading:10861844-Phosphotyrosine, pubmed-meshheading:10861844-Precipitin Tests, pubmed-meshheading:10861844-Protein-Tyrosine Kinases, pubmed-meshheading:10861844-Proto-Oncogene Proteins c-met, pubmed-meshheading:10861844-RNA, Messenger, pubmed-meshheading:10861844-Recombinant Proteins, pubmed-meshheading:10861844-Sialoglycoproteins
pubmed:year
2000
pubmed:articleTitle
Osteopontin-induced, integrin-dependent migration of human mammary epithelial cells involves activation of the hepatocyte growth factor receptor (Met).
pubmed:affiliation
Department of Pathology, University of Western Ontario, London, Canada. atuck@julian.uwo.ca
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't