Source:http://linkedlifedata.com/resource/pubmed/id/10861581
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2000-7-13
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| pubmed:abstractText |
Interleukin (IL)-15 shares immuno-stimulatory properties with IL-2 and is a potent inducer of natural killer (NK) cell function. The major histocompatibility complex (MHC) class I-negative human small cell lung cancer (SCLC) cell line N592, engineered to express a modified IL-15 cDNA (N592/IL-15), secreted biologically active IL-15 (300-500 pg/ml), capable of boosting T-cell proliferation and NK activity 'in vitro'. The effect of IL-15 gene transfer on natural immunity 'in vivo' was assessed by xenotransplants in nude mice and compared with that of the IL-2 gene. N592 cells engineered with IL-2 (N592/IL-2) were promptly rejected, while N592/IL-15 displayed a significant delay in tumour growth and a slightly reduced take rate. However, in NK-depleted nude mice, N592/IL-15 displayed the same growth kinetics as unmodified N592 cells, and N592/IL-2 grew with slightly reduced kinetics. An impressive reactive cell infiltration, consisting mainly of macrophages and granulocytes, was associated with N592/IL-2 tumour rejection, while a more evident recruitment of NK cells was found in N592/IL-15 tumours. In both N592 transfected tumours, we found expression of chemoattractant molecules, such as granulocyte macrophage-colony stimulating factor (GM-CSF) and monocyte chemoattractant protein (MCP)-1, while macrophage inflammatory protein (MIP)-2 was produced by endothelial cells only in N592/IL-2 tumours. In this tumour, very few and severely damaged microvessels were found, while microvessels were numerous in N592/IL-15 tumours. The potent recruitment of NK cells mediated by IL-15 gene transfer suggests its possible therapeutic use in tumours lacking MHC class I.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL2,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte-Macrophage...,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-15,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Macrophage Inflammatory Proteins
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jun
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| pubmed:issn |
0022-3417
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2000 John Wiley & Sons, Ltd.
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| pubmed:issnType |
Print
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| pubmed:volume |
191
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
193-201
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:10861581-Animals,
pubmed-meshheading:10861581-Carcinoma, Small Cell,
pubmed-meshheading:10861581-Chemokine CCL2,
pubmed-meshheading:10861581-Female,
pubmed-meshheading:10861581-Gene Transfer Techniques,
pubmed-meshheading:10861581-Genes, MHC Class I,
pubmed-meshheading:10861581-Graft Rejection,
pubmed-meshheading:10861581-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:10861581-Humans,
pubmed-meshheading:10861581-Immunity, Innate,
pubmed-meshheading:10861581-Interleukin-15,
pubmed-meshheading:10861581-Interleukin-2,
pubmed-meshheading:10861581-Killer Cells, Natural,
pubmed-meshheading:10861581-Lung Neoplasms,
pubmed-meshheading:10861581-Macrophage Inflammatory Proteins,
pubmed-meshheading:10861581-Mice,
pubmed-meshheading:10861581-Mice, Nude,
pubmed-meshheading:10861581-Neoplasm Transplantation,
pubmed-meshheading:10861581-Tumor Cells, Cultured
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| pubmed:year |
2000
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| pubmed:articleTitle |
Dissimilar anti-tumour reactions induced by tumour cells engineered with the interleukin-2 or interleukin-15 gene in nude mice.
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| pubmed:affiliation |
Dipartimento di Oncologia e Neuroscienze, Università di Chieti, Italy.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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