Source:http://linkedlifedata.com/resource/pubmed/id/10861407
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2000-8-23
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| pubmed:abstractText |
A framework for the online optimization of protein induction using green fluorescent protein (GFP)-monitoring technology was developed for high-cell-density cultivation of Escherichia coli. A simple and unstructured mathematical model was developed that described well the dynamics of cloned chloramphenicol acetyltransferase (CAT) production in E. coli JM105 was developed. A sequential quadratic programming (SQP) optimization algorithm was used to estimate model parameter values and to solve optimal open-loop control problems for piecewise control of inducer feed rates that maximize productivity. The optimal inducer feeding profile for an arabinose induction system was different from that of an isopropyl-beta-D-thiogalactopyranoside (IPTG) induction system. Also, model-based online parameter estimation and online optimization algorithms were developed to determine optimal inducer feeding rates for eventual use of a feedback signal from a GFP fluorescence probe (direct product monitoring with 95-minute time delay). Because the numerical algorithms required minimal processing time, the potential for product-based and model-based online optimal control methodology can be realized.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Arabinose,
http://linkedlifedata.com/resource/pubmed/chemical/Chloramphenicol O-Acetyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Isopropyl Thiogalactoside,
http://linkedlifedata.com/resource/pubmed/chemical/Luminescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins
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| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
0006-3592
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2000 John Wiley & Sons, Inc.
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| pubmed:issnType |
Print
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| pubmed:day |
5
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| pubmed:volume |
69
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
275-85
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:10861407-Algorithms,
pubmed-meshheading:10861407-Arabinose,
pubmed-meshheading:10861407-Biomass,
pubmed-meshheading:10861407-Cell Division,
pubmed-meshheading:10861407-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:10861407-Computer Simulation,
pubmed-meshheading:10861407-Escherichia coli,
pubmed-meshheading:10861407-Feedback,
pubmed-meshheading:10861407-Fluorescent Dyes,
pubmed-meshheading:10861407-Gene Expression Regulation, Bacterial,
pubmed-meshheading:10861407-Genes, Reporter,
pubmed-meshheading:10861407-Glucose,
pubmed-meshheading:10861407-Green Fluorescent Proteins,
pubmed-meshheading:10861407-Isopropyl Thiogalactoside,
pubmed-meshheading:10861407-Kinetics,
pubmed-meshheading:10861407-Luminescent Proteins,
pubmed-meshheading:10861407-Models, Biological,
pubmed-meshheading:10861407-Recombinant Fusion Proteins
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| pubmed:year |
2000
|
| pubmed:articleTitle |
Framework for online optimization of recombinant protein expression in high-cell-density Escherichia coli cultures using GFP-fusion monitoring.
|
| pubmed:affiliation |
Center for Agricultural Biotechnology, University of Maryland Biotechnology Institute, College Park 20742, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|