Source:http://linkedlifedata.com/resource/pubmed/id/10858516
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12
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| pubmed:dateCreated |
2000-9-25
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| pubmed:abstractText |
Bothropstoxin-I (BthTX-I) and bothropstoxin-II (BthTX-II) are Lys-49 and Asp-49 phospholipases A(2) (PLA(2)s), respectively, isolated from Bothrops jararacussu venom. Piratoxin-I (PrTX-I) is a Lys-49 PLA(2) isolated from Bothrops pirajai venom. In this study, the ability of BthTX-I, BthTX-II and PrTX-I to recruit leucocytes into the rat pleural cavity and potential mechanisms underlying this effect were investigated. Intrapleural injection of either BthTX-I or PrTX-I (10-100 microg/cavity each) caused a significant leucocyte infiltration at 12 h after injection. The maximal cell migration was observed with the dose of 30 microg/cavity (14.9+/-15.5 and 17.6+/-1. 6x10(6) cells/cavity, respectively). Leucocyte counts consisted mainly of mononuclear cells, but significant amounts of neutrophils and eosinophils were also observed. Intrapleural injection of BthTX-II (10-100 microg/cavity) caused a marked leucocyte infiltration at 6 and 12 h after injection. The maximal response was observed with the dose of 100 microg/cavity (57.3+/-3.4x10(6) cells/cavity, 6 h). The leucocyte counts were mainly composed of neutrophils and mononuclear cells. The treatment of either BthTX-I (30 microg/cavity, 12 h) or BthTX-II (30 microg/cavity, 6 h) with the PLA(2) inhibitor p-bromophenacyl bromide (p-BPB) had no effect on the total and differential leucocyte counts induced by these proteins. The same treatment partially reduced the PrTX-I-induced pleural leucocyte infiltration. In rats depleted of the histamine and 5-hydroxytryptamine (5-HT) stores by chronic treatment with compound 48/80, the total leucocyte counts in response to BthTX-I, BthTX-II and PrTX-I was not significantly affected compared to control animals. In addition, BthTX-I, BthTX-II and PrTX-I (100 microg/ml each) significantly degranulated pleural mast cells in vitro leading to the release of [(14)C]5-hydroxytryptamine ([(14)C]5-HT). p-BPB and heparin (50 IU/ml) significantly reduced the [(14)C]5-HT release induced by these PLA(2)s. Our results demonstrate that BthTX-I, BthTX-II and PrTX-I recruit leucocyte into the pleural cavity of the rat by mechanisms unrelated to enzymatic activity and pleural mast cell degranulation.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/4-bromophenacyl bromide,
http://linkedlifedata.com/resource/pubmed/chemical/Acetophenones,
http://linkedlifedata.com/resource/pubmed/chemical/Crotalid Venoms,
http://linkedlifedata.com/resource/pubmed/chemical/Group II Phospholipases A2,
http://linkedlifedata.com/resource/pubmed/chemical/Histamine,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipases A,
http://linkedlifedata.com/resource/pubmed/chemical/Reptilian Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin,
http://linkedlifedata.com/resource/pubmed/chemical/bothropstoxin,
http://linkedlifedata.com/resource/pubmed/chemical/bothropstoxin II,
http://linkedlifedata.com/resource/pubmed/chemical/p-Methoxy-N-methylphenethylamine,
http://linkedlifedata.com/resource/pubmed/chemical/piratoxin I
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| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
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| pubmed:issn |
0041-0101
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
38
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1773-85
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:10858516-Acetophenones,
pubmed-meshheading:10858516-Animals,
pubmed-meshheading:10858516-Cell Degranulation,
pubmed-meshheading:10858516-Chemotaxis, Leukocyte,
pubmed-meshheading:10858516-Crotalid Venoms,
pubmed-meshheading:10858516-Eosinophils,
pubmed-meshheading:10858516-Group II Phospholipases A2,
pubmed-meshheading:10858516-Histamine,
pubmed-meshheading:10858516-Leukocyte Count,
pubmed-meshheading:10858516-Male,
pubmed-meshheading:10858516-Mast Cells,
pubmed-meshheading:10858516-Neutrophils,
pubmed-meshheading:10858516-Phospholipases A,
pubmed-meshheading:10858516-Pleura,
pubmed-meshheading:10858516-Rats,
pubmed-meshheading:10858516-Rats, Wistar,
pubmed-meshheading:10858516-Reptilian Proteins,
pubmed-meshheading:10858516-Serotonin,
pubmed-meshheading:10858516-p-Methoxy-N-methylphenethylamine
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| pubmed:year |
2000
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| pubmed:articleTitle |
Leucocyte recruitment induced by type II phospholipases A(2) into the rat pleural cavity.
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| pubmed:affiliation |
Department of Pharmacology, Faculty of Medical Sciences, UNICAMP, PO Box 6111, 13081-970, (SP), Campinas, Brazil.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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