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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3-4
|
| pubmed:dateCreated |
1976-12-1
|
| pubmed:abstractText |
A technique for the single step separation of human thymus derived (T) and Bursa equivalent (B) lymphocytes was developed. Density separation of B lymphocytes and T lymphocyte-sheep red blood cell (SRBC) rosettes on Ficoll-Hypaque was modified by pretreatment of the SRBC with 2 aminoethylisothiouronium bromide hydrobromide. This modification yielded significantly purer populations of T and B cells. Up to 15 X 10(7) were separated without increasing contamination allowing for the recovery of B lymphocytes as well as T lymphocytes in sufficient numbers to use in functional assays.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0022-1759
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
12
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
285-8
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:1085784-B-Lymphocytes,
pubmed-meshheading:1085784-Cell Separation,
pubmed-meshheading:1085784-Centrifugation,
pubmed-meshheading:1085784-Erythrocytes,
pubmed-meshheading:1085784-Humans,
pubmed-meshheading:1085784-Immune Adherence Reaction,
pubmed-meshheading:1085784-T-Lymphocytes,
pubmed-meshheading:1085784-beta-Aminoethyl Isothiourea
|
| pubmed:year |
1976
|
| pubmed:articleTitle |
Single step separation of human T and B cells using AET treated srbc rosettes.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|