| pubmed-article:10846054 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10846054 | lifeskim:mentions | umls-concept:C0035736 | lld:lifeskim |
| pubmed-article:10846054 | lifeskim:mentions | umls-concept:C0949930 | lld:lifeskim |
| pubmed-article:10846054 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:10846054 | lifeskim:mentions | umls-concept:C0035668 | lld:lifeskim |
| pubmed-article:10846054 | lifeskim:mentions | umls-concept:C0086222 | lld:lifeskim |
| pubmed-article:10846054 | lifeskim:mentions | umls-concept:C1547530 | lld:lifeskim |
| pubmed-article:10846054 | lifeskim:mentions | umls-concept:C1705733 | lld:lifeskim |
| pubmed-article:10846054 | lifeskim:mentions | umls-concept:C0439581 | lld:lifeskim |
| pubmed-article:10846054 | pubmed:issue | 13 | lld:pubmed |
| pubmed-article:10846054 | pubmed:dateCreated | 2000-8-10 | lld:pubmed |
| pubmed-article:10846054 | pubmed:abstractText | Time course and mutational analyses were used to examine the accumulation in protoplasts of progeny RNAs of the bipartite Crinivirus, Lettuce infectious yellow virus (LIYV; family Closteroviridae). Hybridization analyses showed that simultaneous inoculation of LIYV RNAs 1 and 2 resulted in asynchronous accumulation of progeny LIYV RNAs. LIYV RNA 1 progeny genomic and subgenomic RNAs could be detected in protoplasts as early as 12 h postinoculation (p.i.) and accumulated to high levels by 24 h p.i. The LIYV RNA 1 open reading frame 2 (ORF 2) subgenomic RNA was the most abundant of all LIYV RNAs detected. In contrast, RNA 2 progeny were not readily detected until ca. 36 h p.i. Mutational analyses showed that in-frame stop codons introduced into five of seven RNA 2 ORFs did not affect accumulation of progeny LIYV RNA 1 or RNA 2, confirming that RNA 2 does not encode proteins necessary for LIYV RNA replication. Mutational analyses also supported that LIYV RNA 1 encodes proteins necessary for replication of LIYV RNAs 1 and 2. A mutation introduced into the LIYV RNA 1 region encoding the overlapping ORF 1B and ORF 2 was lethal. However, mutations introduced into only LIYV RNA 1 ORF 2 resulted in accumulation of progeny RNA 1 near or equal to wild-type RNA 1. In contrast, the RNA 1 ORF 2 mutants did not efficiently support the trans accumulation of LIYV RNA 2. Three distinct RNA 1 ORF 2 mutants were analyzed and all exhibited a similar phenotype for progeny LIYV RNA accumulation. These data suggest that the LIYV RNA 1 ORF 2 encodes a trans enhancer for RNA 2 accumulation. | lld:pubmed |
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| pubmed-article:10846054 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10846054 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10846054 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10846054 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10846054 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10846054 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10846054 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:10846054 | pubmed:issn | 0022-538X | lld:pubmed |
| pubmed-article:10846054 | pubmed:author | pubmed-author:RubinMM | lld:pubmed |
| pubmed-article:10846054 | pubmed:author | pubmed-author:CrawfordBB | lld:pubmed |
| pubmed-article:10846054 | pubmed:author | pubmed-author:YehH HHH | lld:pubmed |
| pubmed-article:10846054 | pubmed:author | pubmed-author:FalkB WBW | lld:pubmed |
| pubmed-article:10846054 | pubmed:author | pubmed-author:TianTT | lld:pubmed |
| pubmed-article:10846054 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10846054 | pubmed:volume | 74 | lld:pubmed |
| pubmed-article:10846054 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10846054 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10846054 | pubmed:pagination | 5762-8 | lld:pubmed |
| pubmed-article:10846054 | pubmed:dateRevised | 2010-9-14 | lld:pubmed |
| pubmed-article:10846054 | pubmed:meshHeading | pubmed-meshheading:10846054... | lld:pubmed |
| pubmed-article:10846054 | pubmed:meshHeading | pubmed-meshheading:10846054... | lld:pubmed |
| pubmed-article:10846054 | pubmed:meshHeading | pubmed-meshheading:10846054... | lld:pubmed |
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| pubmed-article:10846054 | pubmed:meshHeading | pubmed-meshheading:10846054... | lld:pubmed |
| pubmed-article:10846054 | pubmed:meshHeading | pubmed-meshheading:10846054... | lld:pubmed |
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| pubmed-article:10846054 | pubmed:meshHeading | pubmed-meshheading:10846054... | lld:pubmed |
| pubmed-article:10846054 | pubmed:meshHeading | pubmed-meshheading:10846054... | lld:pubmed |
| pubmed-article:10846054 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10846054 | pubmed:articleTitle | Asynchronous accumulation of lettuce infectious yellows virus RNAs 1 and 2 and identification of an RNA 1 trans enhancer of RNA 2 accumulation. | lld:pubmed |
| pubmed-article:10846054 | pubmed:affiliation | Department of Plant Pathology, University of California, Davis 95616, USA. | lld:pubmed |
| pubmed-article:10846054 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10846054 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:10846054 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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