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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13
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pubmed:dateCreated |
2000-8-10
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pubmed:abstractText |
Time course and mutational analyses were used to examine the accumulation in protoplasts of progeny RNAs of the bipartite Crinivirus, Lettuce infectious yellow virus (LIYV; family Closteroviridae). Hybridization analyses showed that simultaneous inoculation of LIYV RNAs 1 and 2 resulted in asynchronous accumulation of progeny LIYV RNAs. LIYV RNA 1 progeny genomic and subgenomic RNAs could be detected in protoplasts as early as 12 h postinoculation (p.i.) and accumulated to high levels by 24 h p.i. The LIYV RNA 1 open reading frame 2 (ORF 2) subgenomic RNA was the most abundant of all LIYV RNAs detected. In contrast, RNA 2 progeny were not readily detected until ca. 36 h p.i. Mutational analyses showed that in-frame stop codons introduced into five of seven RNA 2 ORFs did not affect accumulation of progeny LIYV RNA 1 or RNA 2, confirming that RNA 2 does not encode proteins necessary for LIYV RNA replication. Mutational analyses also supported that LIYV RNA 1 encodes proteins necessary for replication of LIYV RNAs 1 and 2. A mutation introduced into the LIYV RNA 1 region encoding the overlapping ORF 1B and ORF 2 was lethal. However, mutations introduced into only LIYV RNA 1 ORF 2 resulted in accumulation of progeny RNA 1 near or equal to wild-type RNA 1. In contrast, the RNA 1 ORF 2 mutants did not efficiently support the trans accumulation of LIYV RNA 2. Three distinct RNA 1 ORF 2 mutants were analyzed and all exhibited a similar phenotype for progeny LIYV RNA accumulation. These data suggest that the LIYV RNA 1 ORF 2 encodes a trans enhancer for RNA 2 accumulation.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10846054-10355756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10846054-10377432,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10846054-10405369,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10846054-10611288,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10846054-11831736,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10846054-2209552,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10846054-7708667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10846054-7793082,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10846054-8021583,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10846054-8234272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10846054-8337819,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10846054-8806497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10846054-8895832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10846054-8895833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10846054-9568985,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10846054-9621048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10846054-9694655,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10846054-9721240
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
74
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5762-8
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pubmed:dateRevised |
2010-9-14
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pubmed:meshHeading |
pubmed-meshheading:10846054-Base Sequence,
pubmed-meshheading:10846054-Chenopodiaceae,
pubmed-meshheading:10846054-Closterovirus,
pubmed-meshheading:10846054-DNA, Viral,
pubmed-meshheading:10846054-Enhancer Elements, Genetic,
pubmed-meshheading:10846054-Lettuce,
pubmed-meshheading:10846054-Molecular Sequence Data,
pubmed-meshheading:10846054-Mutagenesis,
pubmed-meshheading:10846054-RNA, Viral
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pubmed:year |
2000
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pubmed:articleTitle |
Asynchronous accumulation of lettuce infectious yellows virus RNAs 1 and 2 and identification of an RNA 1 trans enhancer of RNA 2 accumulation.
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pubmed:affiliation |
Department of Plant Pathology, University of California, Davis 95616, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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