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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
33
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pubmed:dateCreated |
2000-9-21
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pubmed:abstractText |
Regulation of epithelial Na(+) channel (ENaC) subunit levels by protein kinase C (PKC) was investigated in A6 cells. PKC activation altered ENaC subunit levels, differentially decreasing the levels of both beta and gamma, but not alphaENaC. Temporal regulation of beta and gammaENaC by PKC differed; gammaENaC decreased with a time constant of 3.7 +/- 1.0 h, whereas betaENaC decreased in 13.9 +/- 3. 0 h. Activation of PKC also resulted in a decrease in trans-epithelial Na(+) reabsorption for up to 48 h. PMA activation of PKC resulted in negative feedback inhibition of PKC protein levels beginning within 4 h. Both beta and gammaENaC levels, as well as transport tended toward pretreatment values after 48 h of PMA treatment. PKC inhibitors attenuated the effects of PMA on ENaC subunit levels and Na(+) transport. These results directly show for the first time that PKC differentially regulates ENaC subunit levels by decreasing the levels of beta and gamma but not alphaENaC protein. These results imply a PKC-dependent, long term decrease in Na(+) reabsorption.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Aldosterone,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Epithelial Sodium Channel,
http://linkedlifedata.com/resource/pubmed/chemical/Indoles,
http://linkedlifedata.com/resource/pubmed/chemical/Maleimides,
http://linkedlifedata.com/resource/pubmed/chemical/Naphthalenes,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate,
http://linkedlifedata.com/resource/pubmed/chemical/bisindolylmaleimide I,
http://linkedlifedata.com/resource/pubmed/chemical/calphostin C
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
18
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pubmed:volume |
275
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
25760-5
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:10829029-Aldosterone,
pubmed-meshheading:10829029-Animals,
pubmed-meshheading:10829029-Biological Transport,
pubmed-meshheading:10829029-Blotting, Western,
pubmed-meshheading:10829029-Cell Line,
pubmed-meshheading:10829029-Dose-Response Relationship, Drug,
pubmed-meshheading:10829029-Electrophysiology,
pubmed-meshheading:10829029-Enzyme Activation,
pubmed-meshheading:10829029-Enzyme Inhibitors,
pubmed-meshheading:10829029-Epithelial Sodium Channel,
pubmed-meshheading:10829029-Indoles,
pubmed-meshheading:10829029-Kinetics,
pubmed-meshheading:10829029-Maleimides,
pubmed-meshheading:10829029-Naphthalenes,
pubmed-meshheading:10829029-Protein Kinase C,
pubmed-meshheading:10829029-Protein Structure, Tertiary,
pubmed-meshheading:10829029-RNA, Messenger,
pubmed-meshheading:10829029-Sodium,
pubmed-meshheading:10829029-Sodium Channels,
pubmed-meshheading:10829029-Tetradecanoylphorbol Acetate,
pubmed-meshheading:10829029-Time Factors,
pubmed-meshheading:10829029-Xenopus
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pubmed:year |
2000
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pubmed:articleTitle |
Differential effects of protein kinase C on the levels of epithelial Na+ channel subunit proteins.
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pubmed:affiliation |
Department of Physiology, University of Texas Health Science Center, San Antonio 78229, USA. stockand@UTHSCSA.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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