Linked Life Data

10827951

Source:http://linkedlifedata.com/resource/pubmed/id/10827951

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5470
pubmed:dateCreated
2000-6-8
pubmed:abstractText
We show that transcription factor IIH ERCC3 subunit, the DNA helicase responsible for adenosine triphosphate (ATP)-dependent promoter melting during transcription initiation, does not interact with the promoter region that undergoes melting but instead interacts with DNA downstream of this region. We show further that promoter melting does not change protein-DNA interactions upstream of the region that undergoes melting but does change interactions within and downstream of this region. Our results rule out the proposal that IIH functions in promoter melting through a conventional DNA-helicase mechanism. We propose that IIH functions as a molecular wrench: rotating downstream DNA relative to fixed upstream protein-DNA interactions, thereby generating torque on, and melting, the intervening DNA.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0036-8075
pubmed:author
pubmed:issnType
Print
pubmed:day
26
pubmed:volume
288
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1418-22
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:10827951-Adenosine Triphosphate, pubmed-meshheading:10827951-Base Sequence, pubmed-meshheading:10827951-DNA, pubmed-meshheading:10827951-DNA, Single-Stranded, pubmed-meshheading:10827951-DNA Helicases, pubmed-meshheading:10827951-DNA-Binding Proteins, pubmed-meshheading:10827951-Humans, pubmed-meshheading:10827951-Models, Genetic, pubmed-meshheading:10827951-Molecular Sequence Data, pubmed-meshheading:10827951-Nucleic Acid Conformation, pubmed-meshheading:10827951-Promoter Regions, Genetic, pubmed-meshheading:10827951-Protein Binding, pubmed-meshheading:10827951-RNA Polymerase II, pubmed-meshheading:10827951-Transcription, Genetic, pubmed-meshheading:10827951-Transcription Factor TFIIH, pubmed-meshheading:10827951-Transcription Factors, pubmed-meshheading:10827951-Transcription Factors, TFII
pubmed:year
2000
pubmed:articleTitle
Mechanism of ATP-dependent promoter melting by transcription factor IIH.
pubmed:affiliation
Howard Hughes Medical Institute, Division of Nucleic Acids Enzymology, Department of Biochemistry, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't