Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2000-7-20
pubmed:abstractText
A novel protein kinase (BjCCaBPk) from etiolated Brassica juncea seedlings has been purified and partially characterized. The purified enzyme migrated on SDS/PAGE as a single band with an apparent molecular mass of 43 kDa. The optimum pH for the kinase activity was 8.0. It was stimulated more than sixfold by the protozoa Entamoeba histolytica calcium binding protein EhCaBP (10.5 nM) but not by calmodulin (CaM) when used at equimolar concentration. Moreover the kinase also did not bind CaM-Sepharose. There was neither inhibition of the kinase activity in the presence of W-7 (a CaM antagonist), KN-62 (a specific calcium/CaM kinase inhibitor) and anti-CaM Ig, nor any effect on BjCCaBPk activity of staurosporine (a protein kinase C inhibitor). Furthermore a CaM-kinase specific substrate, syntide-2, proved to be a poor substrate for the BjCCaBPk compared with histone III-S. The phosphorylation of histone III-S involved serine residues. Southern and Northern blot analysis showed the presence of EhCaBP homologues in Brassica. The data suggest that BjCCaBPk may be a novel protein kinase with an affinity towards a calcium binding protein like EhCaBP.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/1-(5-Isoquinolinesulfonyl)-2-Methylp..., http://linkedlifedata.com/resource/pubmed/chemical/Calcium, http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Calmodulin, http://linkedlifedata.com/resource/pubmed/chemical/Chelating Agents, http://linkedlifedata.com/resource/pubmed/chemical/Egtazic Acid, http://linkedlifedata.com/resource/pubmed/chemical/Histones, http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G, http://linkedlifedata.com/resource/pubmed/chemical/KN 62, http://linkedlifedata.com/resource/pubmed/chemical/Peptides, http://linkedlifedata.com/resource/pubmed/chemical/Phosphoserine, http://linkedlifedata.com/resource/pubmed/chemical/Plant Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Protozoan Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Staurosporine, http://linkedlifedata.com/resource/pubmed/chemical/Sulfonamides, http://linkedlifedata.com/resource/pubmed/chemical/W 7, http://linkedlifedata.com/resource/pubmed/chemical/calcium-binding protein, Entamoeba..., http://linkedlifedata.com/resource/pubmed/chemical/syntide-2
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0014-2956
pubmed:author
pubmed:issnType
Print
pubmed:volume
267
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3181-9
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:10824102-1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine, pubmed-meshheading:10824102-Animals, pubmed-meshheading:10824102-Brassica, pubmed-meshheading:10824102-Calcium, pubmed-meshheading:10824102-Calcium Signaling, pubmed-meshheading:10824102-Calcium-Binding Proteins, pubmed-meshheading:10824102-Calmodulin, pubmed-meshheading:10824102-Chelating Agents, pubmed-meshheading:10824102-Egtazic Acid, pubmed-meshheading:10824102-Entamoeba histolytica, pubmed-meshheading:10824102-Histones, pubmed-meshheading:10824102-Immunoglobulin G, pubmed-meshheading:10824102-Peptides, pubmed-meshheading:10824102-Phosphorylation, pubmed-meshheading:10824102-Phosphoserine, pubmed-meshheading:10824102-Plant Proteins, pubmed-meshheading:10824102-Protein Kinases, pubmed-meshheading:10824102-Protein Processing, Post-Translational, pubmed-meshheading:10824102-Protozoan Proteins, pubmed-meshheading:10824102-Recombinant Fusion Proteins, pubmed-meshheading:10824102-Staurosporine, pubmed-meshheading:10824102-Sulfonamides
pubmed:year
2000
pubmed:articleTitle
A novel protein kinase from Brassica juncea stimulated by a protozoan calcium binding protein. Purification and partial characterization.
pubmed:affiliation
National Center for Plant Genome Research, Jawaharlal Nehru University Campus, New Delhi, India.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't