Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2000-8-2
pubmed:abstractText
Previous epidemiological evidence suggested that in some instances a vector and/or reservoir is involved in the occurrence and spread of transmissible spongiform encephalopathies (TSEs). In a preliminary study, hay mite preparations from five Icelandic farms with a history of scrapie were injected into mice, and some of these mice became sick after long incubation periods. To confirm that the disease was scrapie, subsequent passages in mice were performed. In addition, the characteristics of the disease process in these passages were assessed and the results compared to those findings with standard scrapie strains. As expected for scrapie, subsequent passages in the same host led to shortened incubation periods compared to those in primary isolate mice, and all mice had spongiform changes in brain. Results were similar for three of four isolates with regard to clinical manifestations, the incubation periods in mice of the three scrapie incubation-period genotypes (s7s7, s7p7, p7p7), and the PrPSc Western blot (WB) pattern. The characteristics of the fourth isolate were markedly different from the other three isolates with regard to these parameters. Comparison of the characteristics of standard mouse-adapted scrapie strains and the four isolates revealed differences; these differences were particularly pronounced for the fourth isolate.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1355-0284
pubmed:author
pubmed:issnType
Print
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
137-44
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10822327-Animal Feed, pubmed-meshheading:10822327-Animals, pubmed-meshheading:10822327-Arachnid Vectors, pubmed-meshheading:10822327-Brain, pubmed-meshheading:10822327-Crosses, Genetic, pubmed-meshheading:10822327-Food Parasitology, pubmed-meshheading:10822327-Genetic Predisposition to Disease, pubmed-meshheading:10822327-Genotype, pubmed-meshheading:10822327-Iceland, pubmed-meshheading:10822327-Injections, pubmed-meshheading:10822327-Injections, Intraperitoneal, pubmed-meshheading:10822327-Mice, pubmed-meshheading:10822327-Mice, Inbred C57BL, pubmed-meshheading:10822327-Mice, Inbred Strains, pubmed-meshheading:10822327-Mites, pubmed-meshheading:10822327-PrPSc Proteins, pubmed-meshheading:10822327-Scrapie, pubmed-meshheading:10822327-Sheep, pubmed-meshheading:10822327-Time Factors, pubmed-meshheading:10822327-Tissue Extracts, pubmed-meshheading:10822327-Vacuoles
pubmed:year
2000
pubmed:articleTitle
Characteristics of scrapie isolates derived from hay mites.
pubmed:affiliation
Department of Virology, NYS Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, Staten Island, New York, NY 10314-6399, USA.
pubmed:publicationType
Journal Article, Comparative Study