|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
2000-7-7
|
|
pubmed:abstractText |
A recombinant expression system was developed to analyse sequence determinants involved in O-glycosylation of proteins in mycobacteria. By expressing peptide sequences corresponding to known glycosylation sites within a chimeric lipoprotein construct, amino acids flanking modified threonine residues were found to have an important influence on glycosylation. The expression system was used to screen mycobacterial sequences selected using a neural network (NetOglyc) trained on eukaryotic O-glycoproteins. Evidence of glycosylation was obtained for eight of 11 proteins tested. The results suggest that sites involved in O-glycosylation of mycobacterial and eukaryotic proteins share similar structural features.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
May
|
|
pubmed:issn |
0014-5793
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
19
|
|
pubmed:volume |
473
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
358-62
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:10818240-Alkaline Phosphatase,
pubmed-meshheading:10818240-Amino Acid Sequence,
pubmed-meshheading:10818240-Bacterial Proteins,
pubmed-meshheading:10818240-Binding Sites,
pubmed-meshheading:10818240-Concanavalin A,
pubmed-meshheading:10818240-DNA, Recombinant,
pubmed-meshheading:10818240-Glycosylation,
pubmed-meshheading:10818240-Lipoproteins,
pubmed-meshheading:10818240-Molecular Sequence Data,
pubmed-meshheading:10818240-Mycobacterium,
pubmed-meshheading:10818240-Neural Networks (Computer),
pubmed-meshheading:10818240-Protein Processing, Post-Translational,
pubmed-meshheading:10818240-Sequence Alignment,
pubmed-meshheading:10818240-Sequence Analysis, DNA
|
|
pubmed:year |
2000
|
|
pubmed:articleTitle |
Analysis of post-translational modification of mycobacterial proteins using a cassette expression system.
|
|
pubmed:affiliation |
Department of Infectious Diseases and Microbiology, Imperial College School of Medicine, St. Mary's Campus, Norfolk Place, W2 1PG, London, UK. jlh@chu-stlouis.fr
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
