|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
2000-7-11
|
|
pubmed:abstractText |
Autonomous 3'-->5'exonucleases are not bound covalently to DNA polymerases but are often involved in replicative complexes. Such exonucleases from rat liver, calf thymus and Escherichia coli (molecular masses of 28+/-2 kDa) are shown to increase more than 10-fold the accuracy of DNA polymerase beta (the most inaccurate mammalian polymerase) from rat liver in the course of reduplication of the primed DNA of bacteriophage phiX174 amber 3 in vitro. The extent of correction increases together with the rise in 3'-->5' exonuclease concentration. Extrapolation of the in vitro DNA replication fidelity to the cellular levels of rat exonuclease and beta-polymerase suggests that exonucleolytic proofreading could augment the accuracy of DNA synthesis by two orders of magnitude. These results are not explained by exonucleolytic degradation of the primers ("no synthesis-no errors"), since similar data are obtained with the use of the primers 15 or 150 nucleotides long in the course of a fidelity assay of DNA polymerases, both alpha and beta, in the presence of various concentrations of 3'-->5' exonuclease.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Apr
|
|
pubmed:issn |
0027-5107
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
28
|
|
pubmed:volume |
459
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
237-42
|
|
pubmed:dateRevised |
2003-11-14
|
|
pubmed:meshHeading |
pubmed-meshheading:10812336-Animals,
pubmed-meshheading:10812336-Bacteriophage phi X 174,
pubmed-meshheading:10812336-Cattle,
pubmed-meshheading:10812336-Chromatin,
pubmed-meshheading:10812336-Chromatography, Gel,
pubmed-meshheading:10812336-Cytosol,
pubmed-meshheading:10812336-DNA Polymerase I,
pubmed-meshheading:10812336-DNA Polymerase beta,
pubmed-meshheading:10812336-DNA Repair,
pubmed-meshheading:10812336-DNA Replication,
pubmed-meshheading:10812336-Deoxyguanine Nucleotides,
pubmed-meshheading:10812336-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:10812336-Escherichia coli,
pubmed-meshheading:10812336-Exodeoxyribonuclease V,
pubmed-meshheading:10812336-Exodeoxyribonucleases,
pubmed-meshheading:10812336-Liver,
pubmed-meshheading:10812336-Male,
pubmed-meshheading:10812336-Molecular Weight,
pubmed-meshheading:10812336-Mutagenicity Tests,
pubmed-meshheading:10812336-Rats,
pubmed-meshheading:10812336-Regression Analysis,
pubmed-meshheading:10812336-Thymus Gland,
pubmed-meshheading:10812336-Transcription, Genetic
|
|
pubmed:year |
2000
|
|
pubmed:articleTitle |
Autonomous 3'-->5' exonucleases can proofread for DNA polymerase beta from rat liver.
|
|
pubmed:affiliation |
Laboratory of DNA Biosynthesis, Division of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute of the Russia Academy of Sciences, Gatchina, Leningrad, Russia.
|
|
pubmed:publicationType |
Journal Article
|
