Linked Life Data

10811645

Source:http://linkedlifedata.com/resource/pubmed/id/10811645

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
32
pubmed:dateCreated
2000-9-14
pubmed:databankReference
pubmed:abstractText
We have cloned a novel pancreatic beta cell and neuroendocrine cell-specific calcium-binding protein termed secretagogin. The cDNA obtained by immunoscreening a human pancreatic cDNA library using the recently described murine monoclonal antibody D24 contains an open reading frame of 828 base pairs. This codes for a cytoplasmic protein with six putative EF finger hand calcium-binding motifs. The gene could be localized to chromosome 6 by alignment with GenBank genomic sequence data. Northern blot analysis demonstrated abundant expression of this protein in the pancreas and to a lesser extent in the thyroid, adrenal medulla, and cortex. In addition it was expressed in scant quantity in the gastrointestinal tract (stomach, small intestine, and colon). Thyroid tissue expression of secretagogin was restricted to C-cells. Using a sandwich capture enzyme-linked immunosorbent assay with a detection limit of 6.5 pg/ml, considerable amounts of constitutively secreted protein could be measured in tissue culture supernatants of stably transfected RIN-5F and dog insulinoma (INS-H1) cell clones; however, in stably transfected Jurkat cells, the protein was only secreted upon CD3 stimulation. Functional analysis of transfected cell lines expressing secretagogin revealed an influence on calcium flux and cell proliferation. In RIN-5F cells, the antiproliferative effect is possibly due to secretagogin-triggered down-regulation of substance P transcription.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
275
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
24740-51
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10811645-Amino Acid Sequence, pubmed-meshheading:10811645-Animals, pubmed-meshheading:10811645-Base Sequence, pubmed-meshheading:10811645-Calcium, pubmed-meshheading:10811645-Calcium-Binding Protein, Vitamin D-Dependent, pubmed-meshheading:10811645-Calcium-Binding Proteins, pubmed-meshheading:10811645-Chromosome Mapping, pubmed-meshheading:10811645-Chromosomes, Human, Pair 6, pubmed-meshheading:10811645-Cloning, Molecular, pubmed-meshheading:10811645-Dogs, pubmed-meshheading:10811645-Gene Library, pubmed-meshheading:10811645-Humans, pubmed-meshheading:10811645-Insulinoma, pubmed-meshheading:10811645-Islets of Langerhans, pubmed-meshheading:10811645-Jurkat Cells, pubmed-meshheading:10811645-Molecular Sequence Data, pubmed-meshheading:10811645-Open Reading Frames, pubmed-meshheading:10811645-Pancreas, pubmed-meshheading:10811645-Pancreatic Neoplasms, pubmed-meshheading:10811645-Recombinant Proteins, pubmed-meshheading:10811645-Sequence Alignment, pubmed-meshheading:10811645-Sequence Homology, Amino Acid, pubmed-meshheading:10811645-Thyroid Gland, pubmed-meshheading:10811645-Transfection, pubmed-meshheading:10811645-Tumor Cells, Cultured
pubmed:year
2000
pubmed:articleTitle
Cloning and expression of secretagogin, a novel neuroendocrine- and pancreatic islet of Langerhans-specific Ca2+-binding protein.
pubmed:affiliation
Department of Medicine III, Department of Dermatology, and Department of Clinical Pathology, University of Vienna, A-1090 Vienna, Austria. ludwig.wagner@akh-wien.ac.at
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't