Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
41
pubmed:dateCreated
2000-11-13
pubmed:abstractText
Overexpression of the tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) receptors, TRAIL-R1 and TRAIL-R2, induces apoptosis and activation of NF-kappaB in cultured cells. In this study, we have demonstrated differential signaling capacities by both receptors using either epitope-tagged soluble TRAIL (sTRAIL) or sTRAIL that was cross-linked with a monoclonal antibody. Interestingly, sTRAIL was sufficient for induction of apoptosis only in cell lines that were killed by agonistic TRAIL-R1- and TRAIL-R2-specific IgG preparations. Moreover, in these cell lines interleukin-6 secretion and NF-kappaB activation were induced by cross-linked or non-cross-linked anti-TRAIL, as well as by both receptor-specific IgGs. However, cross-linking of sTRAIL was required for induction of apoptosis in cell lines that only responded to the agonistic anti-TRAIL-R2-IgG. Interestingly, activation of c-Jun N-terminal kinase (JNK) was only observed in response to either cross-linked sTRAIL or anti-TRAIL-R2-IgG even in cell lines where both receptors were capable of signaling apoptosis and NF-kappaB activation. Taken together, our data suggest that TRAIL-R1 responds to either cross-linked or non-cross-linked sTRAIL which signals NF-kappaB activation and apoptosis, whereas TRAIL-R2 signals NF-kappaB activation, apoptosis, and JNK activation only in response to cross-linked TRAIL.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal, http://linkedlifedata.com/resource/pubmed/chemical/Apoptosis Regulatory Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G, http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6, http://linkedlifedata.com/resource/pubmed/chemical/JNK Mitogen-Activated Protein..., http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins, http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases, http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, TNF-Related..., http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Tumor Necrosis Factor, http://linkedlifedata.com/resource/pubmed/chemical/Staphylococcal Protein A, http://linkedlifedata.com/resource/pubmed/chemical/TNF-Related Apoptosis-Inducing..., http://linkedlifedata.com/resource/pubmed/chemical/TNFRSF10A protein, human, http://linkedlifedata.com/resource/pubmed/chemical/TNFRSF10B protein, human, http://linkedlifedata.com/resource/pubmed/chemical/TNFSF10 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
13
pubmed:volume
275
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
32208-13
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:10807904-Antibodies, Monoclonal, pubmed-meshheading:10807904-Apoptosis, pubmed-meshheading:10807904-Apoptosis Regulatory Proteins, pubmed-meshheading:10807904-Enzyme Activation, pubmed-meshheading:10807904-Flow Cytometry, pubmed-meshheading:10807904-Humans, pubmed-meshheading:10807904-Immunoglobulin G, pubmed-meshheading:10807904-Interleukin-6, pubmed-meshheading:10807904-JNK Mitogen-Activated Protein Kinases, pubmed-meshheading:10807904-Membrane Glycoproteins, pubmed-meshheading:10807904-Mitogen-Activated Protein Kinases, pubmed-meshheading:10807904-NF-kappa B, pubmed-meshheading:10807904-Receptor Aggregation, pubmed-meshheading:10807904-Receptors, TNF-Related Apoptosis-Inducing Ligand, pubmed-meshheading:10807904-Receptors, Tumor Necrosis Factor, pubmed-meshheading:10807904-Signal Transduction, pubmed-meshheading:10807904-Staphylococcal Protein A, pubmed-meshheading:10807904-TNF-Related Apoptosis-Inducing Ligand, pubmed-meshheading:10807904-Tumor Cells, Cultured, pubmed-meshheading:10807904-Tumor Necrosis Factor-alpha
pubmed:year
2000
pubmed:articleTitle
The tumor necrosis factor-related apoptosis-inducing ligand receptors TRAIL-R1 and TRAIL-R2 have distinct cross-linking requirements for initiation of apoptosis and are non-redundant in JNK activation.
pubmed:affiliation
Institute of Cell Biology and Immunology, University of Stuttgart, Allmandring 31, 70569 Stuttgart, Germany.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't